Building complex glycopeptides: Development of a cysteine-free native chemical ligation protocol

被引：122

作者：

Wu, Bin

Chen, Jiehao

Warren, J. David

Chen, Gong

Hua, Zihao

Danishefsky, Samuel J.

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

[2] Sloan Kettering Inst Canc Res, Bioorgan Chem Lab, New York, NY 10021 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 25期

关键词：

cysteine-free ligation; erythropoietin; glycoproteins; native chemical ligation; peptides;

D O I：

10.1002/anie.200600538

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) The missing link: Reiterative ligations based on non-cysteine and cysteine-based acyl acceptors have been used to prepare synthetic polypeptides and proteins with multiple sites of glycosylation. Highly complex positionally defined glycopolypeptides corresponding to single glycoforms can be formed from simple components. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：4116 / 4125

页数：10

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