Direct electron transfer and electrocatalysis of hemoglobin adsorbed on mesoporous carbon through layer-by-layer assembly

被引：113

作者：

Feng, Jiu-Ju ^{[1
]}

Xu, Jing-Juan ^{[1
]}

Chen, Hong-Yuan ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 08期

基金：

中国国家自然科学基金;

关键词：

mesoporous carbon; layer-by-layer assembly; hemoglobin; direct electron transfer;

D O I：

10.1016/j.bios.2006.07.022

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Using chitosan as an effective linker between CMK-3 and glassy carbon electrode surface, {Hb/CMK-3}(n) multilayer film-modified electrodes were constructed through layer-by-layer assembly. The morphology of thus-formed f Hb/CMK-3 I,, film was characterized by scanning electron micrographs, and the interaction of hemoglobin (Hb) with CMK-3 was studied by UV-vis spectroscopy and electrochemical methods. Under optimal conditions, {Hb/CMK-3}(6) film showed a couple of stable and well-defined redox peaks at about -377 and -296 mV in pH 7.0 buffers. Furthermore, the {Hb/CMK-3}(6) film displayed excellent electrocatalysis to the reduction of both H2O2 and 0,. Based on thus-formed film and its direct electron transfer behavior, a novel biosensor was presented for the determination of H2O2 ranging from 1.2 to 57 mu M with the detection limit of 0.6 mu M at S/N = 3. CMK-3 provided a desirable matrix for protein immobilization and biosensor preparation. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1618 / 1624

页数：7

共 32 条

[21] Chemical strategies to design textured materials: From microporous and mesoporous oxides to nanonetworks and hierarchical structures [J].