DNA-encoded chemical libraries

被引：47

作者：

Scheuermann, Joerg ^{[1
]}

Dumelin, Christoph E. ^{[1
]}

Melkko, Samu ^{[1
]}

Neri, Dario ^{[1
]}

机构：

[1] ETH, Inst Pharmaceut Sci, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF BIOTECHNOLOGY | 2006年 / 126卷 / 04期

关键词：

drug discovery; chemical libraries; DNA-encoding; self-assembly; DNA-templated synthesis; affinity-based selections;

D O I：

10.1016/j.jbiotec.2006.05.018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The discovery and development of novel drugs for the multitude of targets originating from functional genomic research is a challenging task. While antibodies can nowadays be raised against virtually any given target using phage-display methodologies, a similar "selection/amplification" approach for the facile discovery of low-molecular weight compounds capable of specific binding to protein targets of choice has so far been lacking. The development of DNA-encoded chemical libraries, combined with suitable selection and high-throughput sequencing strategies, holds promises to fill this gap. Here, we review the latest developments in the field of DNA-encoded chemical libraries, commenting on the challenges and opportunities for the different experimental strategies in this rapidly evolving research area, which may gain importance for the future drug discovery process. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：568 / 581

页数：14

共 64 条

[1] High-throughput X-ray crystallography for drug discovery [J].

Blundell, TL ;

Patel, S .

CURRENT OPINION IN PHARMACOLOGY, 2004, 4 (05) :490-496

[2] Yeast surface display for screening combinatorial polypeptide libraries [J].

Boder, ET ;

Wittrup, KD .

NATURE BIOTECHNOLOGY, 1997, 15 (06) :553-557

[3] ENCODED COMBINATORIAL CHEMISTRY [J].

BRENNER, S ;

LERNER, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5381-5383

[4]

Calderone CT, 2002, ANGEW CHEM INT EDIT, V41, P4104, DOI 10.1002/1521-3773(20021104)41:21<4104::AID-ANIE4104>3.0.CO

[5]

2-O

[6] MAKING ANTIBODY FRAGMENTS USING PHAGE DISPLAY LIBRARIES [J].

CLACKSON, T ;

HOOGENBOOM, HR ;

GRIFFITHS, AD ;

WINTER, G .

NATURE, 1991, 352 (6336) :624-628

[7]

Comess KM, 2004, CURR OPIN DRUG DISC, V7, P411

[8] Highly sensitive in vitro selections for DNA-linked synthetic small molecules with protein binding affinity and specificity [J].

Doyon, JB ;

Snyder, TM ;

Liu, DR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (41) :12372-12373

[9] Selection of streptavidin binders firom a DNA-encoded chemical library [J].

Dumelin, CE ;

Scheuermann, J ;

Melkko, S ;

Neri, D .

BIOCONJUGATE CHEMISTRY, 2006, 17 (02) :366-370

[10] Beyond Rf tagging [J].

Ede, NJ ;

Wu, ZM .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (03) :374-379

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