Phytotoxic activity of Cachrys pungens Jan, a mediterranean species: separation, identification and quantification of potential allelochemicals

被引:25
作者
Araniti, Fabrizio [1 ]
Marrelli, Mariangela [2 ]
Lupini, Antonio [1 ]
Mercati, Francesco [1 ]
Statti, Giancarlo Antonio [2 ]
Abenavoli, Maria Rosa [1 ]
机构
[1] Univ Studi Mediterranea Reggio Calabria, Fac Agr Salita Melissari, Dipartimento AGR, I-89124 Reggio Di Calabria, RC, Italy
[2] Univ Calabria, Dipartimento Farm & Sci Salute & Nutr, I-87030 Arcavacata Di Rende, CS, Italy
关键词
Amaranthus retroflexus; Apiaceae; Bio-herbicide; Lactuca sativa L; Natural products; Root growth; Seed germination; Echinochloa crus-galli; Lolium perenne; Weed management; GROWTH; GERMINATION; ACCUMULATION; FLAVONOIDS; EXTRACTS; RHIZOSPHERE; COUMARINS; PRODUCTS; ALFALFA; STRESS;
D O I
10.1007/s11738-013-1482-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In continuous research for bioactive compounds obtained from plants to use for weed control in sustainable agriculture, the aerial parts of Cachrys pungens Jan (Umbelliferae) were extracted with methanol and then fractionated using hexane, chloroform (CHCl3) and ethyl acetate (AcOEt). The potential phytotoxicity of total methanolic extract and each fraction was assayed in vitro on seed germination and root elongation of lettuce (Lactuca sativa L.) and the most active fractions were assayed on three of the most common weeds (Lolium perenne, Amaranthus retroflexus, Echinochloa crus-galli). Non linear regression that allowed to obtain the ED50 index for both physiological processes was applied. The fraction bioassays indicated the following hierarchy of phytotoxicity for both processes: CHCl3 a parts per thousand yen AcOEt > hexane. Moreover, in the present work was chemically characterized for the first time (through HPTLC) the polar fraction of this species pointing out the high presence of flavonoids and phenolic acids. In particular six of them have been chemically characterized and quantified (naringin, quercetin, catechin, caffeic acid, ferulic acid and gallic acid). These results make C. pungens Jan a potential source of natural compounds employable for an eco-friendly agriculture.
引用
收藏
页码:1071 / 1083
页数:13
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