Simultaneous determination of diazepam and pyridoxine in synthetic mixtures and pharmaceutical formulations using graphical and multivariate calibration-prediction methods

Several methods are reported for the simultaneous determination of diazepam and pyridoxine: two graphical methods (zero-crossing and derivative quotient spectra); and two numerical methods (multiple linear regression and partial least-squares regression). The methods have been applied in the concentration ranges 1.4-4.0 mu g ml(-1) diazepam and 4.0-12.0 mu g ml(-1) pyridoxine. The accuracy and precision of the methods have been determined and they have been validated by analysing synthetic mixtures containing the two drugs in variable proportions. The methods were also applied to the determination of diazepam and pyridoxine in pharmaceutical preparations. The analytical results were quite good in all cases.