Assessing the structural stability of designed β-hairpin peptides in the cytoplasm of live cells

被引:21
作者
Cheng, Zihao [1 ]
Campbell, Robert E. [1 ]
机构
[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada
关键词
biophysical methods; cells; fluorescent probes; FRET; peptides;
D O I
10.1002/cbic.200500540
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A genetically encoded β-hairpin peptide molecular beacon. By exploiting FRET between genetically fused fluorescent protein variants, we have determined the relative stability of a series of β-hairpins both in vivo and in vitro. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:1147 / 1150
页数:4
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