Preparation of a novel sulfated glycopeptide complex and inhibiting L1210 cell lines property in vitro

被引：22

作者：

Ye, LiBin ^{[1
,2
]}

Zhang, JingSong ^{[1
]}

Zhou, Shuai ^{[1
]}

Wang, Sheng ^{[3
]}

Wu, Di ^{[4
]}

Pan, YingJie ^{[4
]}

机构：

[1] Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China

[2] Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

[3] Cent S Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China

[4] Shanghai Fisheries Univ, Coll Food Sci, Shanghai 200090, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2009年 / 77卷 / 02期

关键词：

Ganoderma lucidum; Glycopeptide; Sulfated derivative; Composition; Anti-tumour; GANODERMA-LUCIDUM; STRUCTURAL ELUCIDATION; ANTITUMOR; POLYSACCHARIDE; PURIFICATION; GLUCAN;

D O I：

10.1016/j.carbpol.2008.12.031

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A native glycopeptide, LZ-D-4, was purified from the fruiting bodies of Ganoderma lucidum, and its derivative, LZ-D-9, was prepared using sulfated agent (CSA: Pyr = 1:6). HPLC experiment showed that both of them were homogeneity and had molecular weights of 1.56 x 10(4) and 1.30 x 10(4) Da, respectively. Some properties including amino acid and monosaccharide compositions were investigated for the two compounds, along with anti-tumour bioactivity in vitro. The results indicated that two monosaccharides (Xyl and Ara) were completely destroyed and most of amino acids in LZ-D-4 were broken during sulfated process. Anti-tumour test in vitro showed that both complexes have properties to inhibit growth of L1210 cell lines and have similar bioactivities, but might have different mechanism. Crown Copyright (c) 2009 Published by Elsevier Ltd. All rights reserved.

引用

页码：276 / 279

页数：4

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