Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

被引：13

作者：

Lim, TK ^{[1
]}

Nakamura, N ^{[1
]}

Matsunaga, T ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL,DEPT BIOTECHNOL,KOGANEI,TOKYO 184,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 354卷 / 1-3期

关键词：

flow injection; immunoassay; fluorescence; cation exchange column; antibody; allergen detection;

D O I：

10.1016/S0003-2670(97)00438-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml(-1). Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen. (C) 1997 Elsevier Science B.V.

引用

页码：29 / 34

页数：6

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