Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant:: characterization of the VanD5 operon

被引：33

作者：

Boyd, DA

Kibsey, P

Roscoe, D

Mulvey, MR

机构：

[1] Hlth Canada, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada

[2] Victoria Gen Hosp, Victoria, BC, Canada

[3] Vancouver Gen Hosp, Vancouver, BC, Canada

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2004年 / 54卷 / 03期

关键词：

glycopeptide resistance; van PCR; E; faecium;

D O I：

10.1093/jac/dkh391

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes. Methods: PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing. Results: Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR. Conclusions: E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.

引用

页码：680 / 683

页数：4

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