Synthesis and characterization of thermo- and pH-sensitive block copolymers bearing a biotin group at the poly(ethylene oxide) chain end

被引：14

作者：

Tong, Dajun ^{[1
]}

Yao, Jia ^{[1
]}

Li, Haoran ^{[1
]}

Han, Shijun ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2006年 / 102卷 / 04期

关键词：

H2N-PEO-PDMAEMA; anionic polymerization; block copolymer; biotin; site-specific drug delivery;

D O I：

10.1002/app.24812

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A poly(ethylene oxide)-block-poly(dimethylamino ethyl methacrylate) block copolymer (PEO-b-PDMAEMA) bearing an amino moiety at the PEO chain end was synthesized by a one-pot sequential oxyanionic polymerization of ethylene oxide (EO) and dimethylamino ethyl methacrylate (DMAEMA), followed by a coupling reaction between its PEO amino and a biotin derivative. The polymers were characterized with H-1 NMR spectroscopy and gel permeation chromatography. Activated biotin, biotin-NHS (N-hydroxysucci-nimide), was used to synthesize biotin-PEO-PDMAEMA. In aqueous media, the solubility of the copolymer was temperature- and pH-sensitive. The particle size of the micelle formed from functionalized block copolymers was determined by dynamic light scattering. (c) 2006 Wiley Periodicals, Inc.

引用

页码：3552 / 3558

页数：7

共 38 条

[11] USE OF AVIDIN-BIOTIN INTERACTION IN IMMUNOENZYMATIC TECHNIQUES [J].

GUESDON, JL ;

TERNYNCK, T ;

AVRAMEAS, S .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1979, 27 (08) :1131-1139

[12] Effect of pegylation on pharmaceuticals [J].

Harris, JM ;

Chess, RB .

NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (03) :214-221

[13] Biotin-derivatized poly(L-lysine)-g-poly(ethylene glycol):: A novel polymeric interface for bioaffinity sensing [J].

Huang, NP ;

Vörös, J ;

De Paul, SM ;

Textor, M ;

Spencer, ND .

LANGMUIR, 2002, 18 (01) :220-230

[14] On the origins of morphological complexity in block copolymer surfactants [J].

Jain, S ;

Bates, FS .

SCIENCE, 2003, 300 (5618) :460-464

[15] SELECTIVE RETRIEVAL OF BIOTIN-LABELED CELLS USING IMMOBILIZED AVIDIN [J].

JASIEWICZ, ML ;

SCHOENBERG, DR ;

MUELLER, GC .

EXPERIMENTAL CELL RESEARCH, 1976, 100 (01) :213-217

[16] Preparation and characterization of core-shell polymer particles with protonizable shells prepared by oxyanionic polymerization [J].

Jin, L ;

Deng, YH ;

Hu, JH ;

Wang, CC .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2004, 42 (23) :6081-6088

[17] Synthesis of well-defined comb-like amphiphilic copolymers with protonizable units in the pendent chains:: 2.: Poly(2-(dimethylamino)ethyl methacrylate) grafted poly(methylmethacrylate-co-2-hydroxyethyl methacrylate) copolymers and their association behavior in aqueous solution [J].

Jin, L ;

Liu, P ;

Hu, JH ;

Wang, CC .

POLYMER INTERNATIONAL, 2004, 53 (02) :142-148

[18] Tailoring the active site of chemzymes by using a chemogenetic-optimization procedure: Towards substrate-specific artificial hydrogenases based on the biotin-avidin technology [J].

Klein, G ;

Humbert, N ;

Gradinaru, J ;

Ivanova, A ;

Gilardoni, F ;

Rusbandi, UE ;

Ward, TR .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (47) :7764-7767

[19] Biological-synthetic hybrid block copolymers: Combining the best from two worlds [J].

Klok, HA .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 2005, 43 (01) :1-17

[20] Latex syntheses using novel tertiary amine methacrylate-based macromonomers prepared by oxyanionic polymerization [J].

Lascelles, SF ;

Malet, F ;

Mayada, R ;

Billingham, NC ;

Armes, SP .

MACROMOLECULES, 1999, 32 (08) :2462-2471

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