A simple 3-step algorithm for improved laboratory detection of Clostridium difficile toxin without the need for tissue culture cytotoxicity neutralization assays

被引：14

作者：

Sharp, Susan E. ^{[1
]}

Ivie, W. Michael ^{[1
]}

Buckles, Misti R. ^{[1
]}

Coover, Diane M. ^{[1
]}

Pohl, Julie C. ^{[1
]}

Hatcher, Patricia A. ^{[1
]}

机构：

[1] Kaiser Permanente NW, Dept Pathol, Portland, OR 97230 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 64卷 / 03期

关键词：

Clostridium difficile; Simple 3-step algorithm; Toxin testing; ANTIGEN;

D O I：

10.1016/j.diagmicrobio.2009.03.009

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

This report describes a 3-step algorithm for the detection of Clostridium difficile using an enzyme immunoassay for glutamate dehydrogenase (GDH) antigen, a lateral flow assay for C. difficile toxin, and anaerobic broth culture. This method was found to detect more toxin-containing stool samples than the use of a combination GDH antigen plus toxin A/B testing alone. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：344 / 346

页数：3

共 4 条

[1] Rapid and reliable diagnostic algorithm for detection of Clostridium difficile [J].

Fenner, Lukas ;

Widmer, Andreas F. ;

Goy, Gisela ;

Rudin, Sonja ;

Frei, Reno .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) :328-330

[2] Is a two-step glutamate dehyrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin a and B enzyme immunoassay for laboratory detection of Clostridium difficile? [J].

Gilligan, Peter H. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (04) :1523-1525

[3] Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile [J].

Reller, Megan E. ;

Lema, Clara A. ;

Perl, Trish M. ;

Cai, Mian ;

Ross, Tracy L. ;

Speck, Kathleen A. ;

Carroll, Karen C. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (11) :3601-3605

[4] Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin [J].

Ticehurst, JR ;

Aird, DZ ;

Dam, LM ;

Borek, AP ;

Hargrove, JT ;

Carroll, KC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (03) :1145-1149

← 1 →