Multiple Wnts are involved in Hydra organizer formation and regeneration

被引:217
作者
Lengfeld, Tobias [3 ]
Watanabe, Hiroshi [3 ]
Simakov, Oleg [3 ]
Lindgens, Dirk [1 ,2 ]
Gee, Lydia [1 ,2 ]
Law, Lee [1 ,2 ]
Schmidt, Heiko A. [4 ,5 ,6 ,7 ]
Oezbek, Suat [3 ]
Bode, Hans [1 ,2 ]
Holstein, Thomas W. [3 ]
机构
[1] Univ Calif Irvine, Dept Dev & Cell Biol, Irvine, CA 92697 USA
[2] Univ Calif Irvine, Ctr Dev Biol, Irvine, CA 92697 USA
[3] Univ Heidelberg, Dept Mol Evolut & Genom, D-69120 Heidelberg, Germany
[4] CIBIV, A-1030 Vienna, Austria
[5] MFPL, A-1030 Vienna, Austria
[6] Med Univ, Univ Vet Med, Vienna, Austria
[7] Univ Vienna, Vienna, Austria
基金
美国国家科学基金会;
关键词
Wnt signaling; Organizer; Axis formation; Regeneration; Cnidaria; Hydra; SIGNALING PATHWAY; GENE FAMILY; DEVELOPMENTAL MECHANISMS; HEAD REGENERATION; XENOPUS EMBRYOS; AXIS FORMATION; BETA-CATENIN; TRANSPLANTATION PHENOMENA; DICTYOSTELIUM-DISCOIDEUM; GASTRULATION MOVEMENTS;
D O I
10.1016/j.ydbio.2009.02.004
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Wnt genes and beta-Catenin signaling are involved in axial patterning processes in vertebrate embryogenesis in setting up the Spemann-Mangold organizer in amphibian embryos. An organizer with a similar function is present in the hypostome of an adult Hydra polyp. Previously, a Hydra ortholog of Wnt3 (HyWnt3), which is expressed in the hypostome, has been described. Here, ten additional Hydra Wnt genes have been identified. Of these, six (HyWnt1, -7, -9/10a, -9/10c, -11, and -16) are expressed in the adult hypostome. And, as is HyWnt3, these six Wnt genes are also expressed when a new head organizer is formed during head regeneration and bud formation. The kinetics of Wnt gene expressions during head regeneration suggests that a cascade of consecutive Wnt activation accompanies regeneration, and HyWnt3 begins this cascade. Recombinant HyWnt3 protein induced body column tissue to undergo head formation. It also increased the head formation capacity in the head regeneration-deficient mutant Strain reg-16 to that of wild-type strains. In addition our data reveal striking similarities in the molecular basis of the organizer in Hydra and axis polarization in chordates (e.g. Spemann's organizer) as well as it's role in regeneration suggesting a conserved function of Wnt signaling in setting up this ancient metazoan signaling center. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:186 / 199
页数:14
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