Determination of single-stranded oligodeoxynucleotides by capillary gel electrophoresis with laser induced fluorescence and on column derivatization

被引：26

作者：

Reyderman, L ^{[1
]}

Stavchansky, S ^{[1
]}

机构：

[1] UNIV TEXAS,COLL PHARM,DIV PHARMACEUT,AUSTIN,TX 78712

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 755卷 / 02期

关键词：

derivatization; electrophoresis; OliGreen(TM) reagent; oligonucleotides; dyes;

D O I：

10.1016/S0021-9673(96)00605-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

OliGreen(TM) reagent, a new dye for complexing of oligonucleotides of low molecular mass was successfully utilized for their quantitative analysis using on column derivatization and capillary gel electrophoresis with laser induced fluorescence. Method optimization for the precise, accurate and reproducible quantitation of low-molecular-mass oligonucleotides from aqueous matrices is described.

引用

收藏

页码：271 / 280

页数：10

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