A unique and universal molecular barcode array

被引：83

作者：

Pierce, Sarah E.

Fung, Eula L.

Jaramillo, Daniel F.

Chu, Angela M.

Davis, Ronald W.

Nislow, Corey

Giaever, Guri

机构：

[1] Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA

[2] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA

[4] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5S 3E1, Canada

[5] Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Fac Pharm, Toronto, ON M5S 3E1, Canada

来源：

NATURE METHODS | 2006年 / 3卷 / 08期

关键词：

D O I：

10.1038/NMETH905

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.

引用

页码：601 / 603

页数：3

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