Bottom-Up Proteomics

被引：13

作者：

Armirotti, Andrea ^{[1
]}

机构：

[1] Univ Genoa, Dept Expt Med, Biochem Sect, Genoa 7, Italy

来源：

CURRENT ANALYTICAL CHEMISTRY | 2009年 / 5卷 / 02期

关键词：

TOF-MASS-SPECTROMETRY; COLLISION-INDUCED DECOMPOSITION; ELECTRON-TRANSFER DISSOCIATION; GAS-PHASE ION; PROTEIN IDENTIFICATION; CONTAINING PEPTIDES; SEQUENCE-ANALYSIS; LASER DESORPTION; MOBILE PROTON; MALDI-TOF/TOF;

D O I：

10.2174/157341109787846117

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, the "bottom-up" protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The state-of-the-art of these technique is outlined along with several recent applications, such as MSn data acquisition and chemical derivatization of peptides.

引用

页码：116 / 130

页数：15

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