Modulating activity of fullerol C60(OH)22 on doxorubicin-induced cytotoxicity

被引:113
作者
Bogdanovic, G
Kojic, V
Dordevic, A
Canadanovic-Brunet, J
Vojinovic-Miloradov, M
Baltic, VV
机构
[1] Inst Oncol Sremska Kamenica, YU-21204 Sremska Kamenica, Serbia
[2] Univ Novi Sad, Fac Sci, YU-21000 Novi Sad, Serbia
[3] Univ Novi Sad, Fac Technol, YU-21000 Novi Sad, Serbia
关键词
doxorubicin; fullerol (non-MeSH); tumor cell; cultured; toxicity tests; hydroxyl radical; free radical scavengers;
D O I
10.1016/j.tiv.2004.02.010
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Paper presents the effects of the newly synthesized fullerol C-60(OH)(22) on the growth of tumor cells in vitro and its modulating activity on doxorubicin (DOX)-induced cytotoxicity in human breast cancer cell lines. Cell growth inhibition was evaluated by tetrazolium colorimetric WST1 assay. Electron spin resonance (ESR) "trapping" method was used to investigate OH-radical scavenger activity of fullerol during Fenton's reaction. At a range of nanomolar concentrations fullerol induced cell growth inhibition, which was cell line, dose and time dependent. Fullerol also strongly suppressed DOX-induced cytotoxicity at all concentrations regardless the time of fullerol addition. Proanthocyanidins added as single agent to MCF-7 cell culture for 48 h induced low growth inhibition but in combination with DOX strongly decreased DOX cytotoxicity. Fullerol was found to be a potent hydroxyl radical scavenger: the relative intensity of ESR signals of DMPO-hydroxyl radical (DMPO-OH) spin adduct decreased by 88% in the presence of 0.5 mug/ml of fullerol. The obtained results suggest that antiproliferative effect of the fullerol and its protective effect on DOX-induced cytotoxicity might be mediated through hydroxyl-radical scavenger activity of C-60(OH)(22). (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:629 / 637
页数:9
相关论文
共 61 条
[1]  
[Anonymous], ARCH ONCOL
[2]  
Bisaglia M, 2000, J NEUROCHEM, V74, P1197
[3]   Antimycobacterial activity of ionic fullerene derivatives [J].
Bosi, S ;
Da Ros, T ;
Castellano, S ;
Banfi, E ;
Prato, M .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2000, 10 (10) :1043-1045
[4]   CELLULARLY GENERATED ACTIVE OXYGEN SPECIES AND HELA-CELL PROLIFERATION [J].
BURDON, RH ;
GILL, V .
FREE RADICAL RESEARCH COMMUNICATIONS, 1993, 19 (03) :203-213
[5]   In vivo studies of fullerene-based materials using endohedral metallofullerene radiotracers [J].
Cagle, DW ;
Kennel, SJ ;
Mirzadeh, S ;
Alford, JM ;
Wilson, LJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (09) :5182-5187
[6]  
CANADANOVICBRUN.JM, 1998, ZADUZBINA ANDREJEVIC, P58
[7]  
CHAING YC, 1992, J CHEM SOC CHEM COMM, P1791
[8]   Antigenicity of fullerenes: Antibodies specific for fullerenes and their characteristics [J].
Chen, BX ;
Wilson, SR ;
Das, M ;
Coughlin, DJ ;
Erlanger, BF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10809-10813
[9]   Synthesis and characterization of fullerol derived from C60n- precursors [J].
Chen, Y ;
Cai, RF ;
Chen, SM ;
Huang, ZE .
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, 2001, 62 (05) :999-1001
[10]   Structures and stabilities of C60(OH)4 and C60(OH)6 fullerenols [J].
Chen, ZF ;
Ma, KQ ;
Wang, GC ;
Zhao, XZ ;
Tang, AC .
JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM, 2000, 498 :227-232