Self-assembly of a nanoscale DNA box with a controllable lid

被引：1233

作者：

Andersen, Ebbe S. ^{[1
,2
,3
]}

Dong, Mingdong ^{[1
,2
,4
]}

Nielsen, Morten M. ^{[1
,2
,3
]}

Jahn, Kasper ^{[1
,2
,3
]}

Subramani, Ramesh ^{[1
,2
,4
]}

Mamdouh, Wael ^{[1
,2
,4
]}

Golas, Monika M. ^{[5
,8
]}

Sander, Bjoern ^{[6
,8
]}

Stark, Holger ^{[8
,9
]}

Oliveira, Cristiano L. P. ^{[2
,7
]}

Pedersen, Jan Skov ^{[2
,7
]}

Birkedal, Victoria ^{[2
]}

Besenbacher, Flemming ^{[1
,2
,4
]}

Gothelf, Kurt V. ^{[1
,2
,7
]}

Kjems, Jorgen ^{[1
,2
,3
]}

机构：

[1] Aarhus Univ, Danish Natl Res Fdn, Ctr DNA Nanotechnol, DK-8000 Aarhus, Denmark

[2] Aarhus Univ, Interdisciplinary Nanosci Ctr, DK-8000 Aarhus, Denmark

[3] Aarhus Univ, Dept Mol Biol, DK-8000 Aarhus, Denmark

[4] Aarhus Univ, Dept Phys & Astron, DK-8000 Aarhus, Denmark

[5] Aarhus Univ, Water & Salt Res Ctr, Inst Anat, DK-8000 Aarhus, Denmark

[6] Aarhus Univ, Stereol & EM Res Lab, DK-8000 Aarhus, Denmark

[7] Aarhus Univ, Dept Chem, DK-8000 Aarhus, Denmark

[8] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

[9] Univ Gottingen, Gottingen Ctr Mol Biol, D-37077 Gottingen, Germany

来源：

NATURE | 2009年 / 459卷 / 7243期

基金：

新加坡国家研究基金会;

关键词：

SMALL-ANGLE SCATTERING; NANOSTRUCTURES; JUNCTIONS; ALIGNMENT;

D O I：

10.1038/nature07971

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The unique structural motifs and self-recognition properties of DNA can be exploited to generate self-assembling DNA nanostructures of specific shapes using a 'bottom-up' approach(1). Several assembly strategies have been developed for building complex three-dimensional (3D) DNA nanostructures(2-8). Recently, the DNA 'origami' method was used to build two-dimensional addressable DNA structures of arbitrary shape(9) that can be used as platforms to arrange nanomaterials with high precision and specificity(9-13). A long-term goal of this field has been to construct fully addressable 3D DNA nanostructures(14,15). Here we extend the DNA origami method into three dimensions by creating an addressable DNA box 42 x 36 x 36 nm(3) in size that can be opened in the presence of externally supplied DNA 'keys'. We thoroughly characterize the structure of this DNA box using cryogenic transmission electron microscopy, small-angle X-ray scattering and atomic force microscopy, and use fluorescence resonance energy transfer to optically monitor the opening of the lid. Controlled access to the interior compartment of this DNA nanocontainer could yield several interesting applications, for example as a logic sensor for multiple-sequence signals or for the controlled release of nanocargos.

引用

页码：73 / U75

页数：5

共 30 条

[11] Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nuclelic acids [J].