Selective determination of quinones by high-performance liquid chromatography with on-line post column ultraviolet irradiation and peroxyoxalate chemiluminescence detection

被引：32

作者：

Ahmed, Sameh ^{[1
]}

Fujii, Shuu ^{[1
]}

Kishikawa, Naoya ^{[1
]}

Ohba, Yoshihito ^{[1
]}

Nakashima, Kenichiro ^{[1
]}

Kuroda, Naotaka ^{[1
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Course Pharmaceut Sci, Nagasaki 8528521, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1133卷 / 1-2期

关键词：

peroxyoxalate chemiluminescence detection; quinones; UV irradiation; HPLC; hydrogen peroxide;

D O I：

10.1016/j.chroma.2006.07.078

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new HPLC method was developed for the simultaneous determination of quinones with peroxyoxalate chemiluminescence (PO-CL) detection following on-line UV irradiation. Quinones [i.e., 1,2-naphthoquinone, 1,4-naphthoquinone, 9,10-anthraquinone, 9,10-phenanthrenequinone] were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide and a fluorescent product that were determined via PO-CL detection. Generation of hydrogen peroxide from quinones with on-line UV irradiation was confirmed using flow injection analysis (FIA) system whereby incorporating an enzyme column reactor immobilized with catalase. Moreover, the structure of the produced fluorophore was confirmed using LC-MS, IR, and H-1 NMR. Afterwards, the conditions for UV irradiation and PO-CL detection were optimized. The separation of four quinones by HPLC was accomplished isocratically on an ODS column within 25 min. The detection limits (signal-to-noise ratio = 3) were 6.0 pmol/injection for 1,2-naphthoquinone, 4.4 pmol/injection for 1,4-naphthoquinone, 0.2 pmol/injection for 9, 10-anthraquinone, and 0.45 pmol/injection for 9, 10-phenanthrenequinone. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：76 / 82

页数：7

共 27 条

[11] Inhibition of nitric oxide formation by neuronal nitric oxide synthase by quinones: Nitric oxide synthase as a quinone reductase [J].