Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice
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作者:
Chujo, Tetsuya
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Chujo, Tetsuya
[1
]
Miyamoto, Koji
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Teikyo Univ, Dept Biosci, Utsunomiya, Tochigi, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Miyamoto, Koji
[1
,3
]
Ogawa, Satoshi
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Ogawa, Satoshi
[1
]
Masuda, Yuka
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Masuda, Yuka
[1
]
Shimizu, Takafumi
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Shimizu, Takafumi
[1
]
Kishi-Kaboshi, Mitsuko
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Natl Inst Agrobiol Sci, Genetically Modified Organism Res Ctr, Tsukuba, Ibaraki, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Kishi-Kaboshi, Mitsuko
[2
]
Takahashi, Akira
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Natl Inst Agrobiol Sci, Genetically Modified Organism Res Ctr, Tsukuba, Ibaraki, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Takahashi, Akira
[2
]
Nishizawa, Yoko
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Natl Inst Agrobiol Sci, Genetically Modified Organism Res Ctr, Tsukuba, Ibaraki, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Nishizawa, Yoko
[2
]
Minami, Eiichi
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Natl Inst Agrobiol Sci, Genetically Modified Organism Res Ctr, Tsukuba, Ibaraki, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Minami, Eiichi
[2
]
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Nojiri, Hideaki
[1
]
Yamane, Hisakazu
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Teikyo Univ, Dept Biosci, Utsunomiya, Tochigi, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Yamane, Hisakazu
[1
,3
]
Okada, Kazunori
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Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, JapanUniv Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
Okada, Kazunori
[1
]
机构:
[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo, Japan
[2] Natl Inst Agrobiol Sci, Genetically Modified Organism Res Ctr, Tsukuba, Ibaraki, Japan
[3] Teikyo Univ, Dept Biosci, Utsunomiya, Tochigi, Japan
WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.