Improved Human Mesenchymal Stem Cell Isolation

被引：22

作者：

Chan, Tzu-Min ^{[1
,2
]}

Harn, Horng-Jyh ^{[3
,4
]}

Lin, Hui-Ping ^{[5
]}

Chou, Pei-Wen ^{[2
,6
]}

Chen, Julia Yi-Ru ^{[6
]}

Ho, Tsung-Jung ^{[7
,8
,9
]}

Chiou, Tzyy-Wen ^{[10
,11
]}

Chuang, Hong-Meng ^{[2
,12
]}

Chiu, Shao-Chih ^{[1
,13
]}

Chen, Yen-Chung ^{[5
]}

Yen, Ssu-Yin ^{[10
,11
]}

Huang, Mao-Hsuan ^{[3
,12
]}

Liang, Bing-Chiang ^{[14
,15
]}

Lin, Shinn-Zong ^{[1
,13
,16
,17
]}

机构：

[1] China Med Univ Hosp, Ctr Neuropsychiat, Taichung, Taiwan

[2] Everfront Biotech Inc, New Taipei City, Taiwan

[3] China Med Univ, Dept Med, Taichung, Taiwan

[4] China Med Univ Hosp, Dept Pathol, Taichung, Taiwan

[5] Natl Hlth Res Inst, Inst Cellular & Syst Med, Miaoli, Taiwan

[6] Guang Li Biomed Inc, New Taipei City, Taiwan

[7] China Med Univ, Sch Chinese Med, Taichung, Taiwan

[8] China Med Univ, Beigang Hosp, Div Chinese Med, Beigang, Yunlin County, Taiwan

[9] China Med Univ, Div Chinese Med, Tainan Municipal An Nan Hosp, Tainan, Taiwan

[10] Natl Dong Hwa Univ, Dept Life Sci, Hualien, Taiwan

[11] Natl Dong Hwa Univ, Grad Inst Biotechnol, Hualien, Taiwan

[12] Natl Chung Hsing Univ Taichung, Dept Life Sci, Taichung, Taiwan

[13] China Med Univ, Grad Inst Immunol, Taichung, Taiwan

[14] Taipei Vet Gen Hosp, Dept Emergency Med, Taipei, Taiwan

[15] Natl Yang Ming Univ, Sch Med, Taipei, Taiwan

[16] China Med Univ, Beigang Hosp, Dept Neurosurg, Yunlin, Taiwan

[17] China Med Univ, Dept Neurosurg, Tainan Municipal An Nan Hosp, Tainan, Taiwan

来源：

CELL TRANSPLANTATION | 2014年 / 23卷 / 4-5期

关键词：

Human mesenchymal stem cells (hMSCs); Improved isolation of hMSCs; Liver progenitors; Mechanical dissociation lipoaspirate; Adipose-derived cell; Novel marrow filter device; Clot spots; Bone marrow-derived mesenchymal stem cells (BMMSCs); EX-VIVO EXPANSION; BONE-MARROW-TRANSPLANTATION; CLINICAL-GRADE PRODUCTION; STROMAL CELLS; UMBILICAL-CORD; IN-VITRO; PROGENITOR CELLS; OSTEOGENESIS IMPERFECTA; ONCOLYTIC ADENOVIRUS; ADIPOSE-TISSUE;

D O I：

10.3727/096368914X678292

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Human mesenchymal stem cells (hMSCs) are currently available for a range of applications and benefits and have become a good material for regenerative medicine, tissue engineering, and disease therapy. Before ex vivo expansion, isolation and characterization of primary hMSCs from peripheral tissues are key steps for obtaining adequate materials for clinical application. The proportion of peripheral stem cells is very low in surrounding tissues and organs; thus the recovery ratio will be a limiting factor. In this review, we summarized current common methods used to isolate peripheral stem cells, as well as the new insights revealed to improve the quantity of stem cells and their sternness. These strategies offer alternative ways to acquire hMSCs in a convenient and/or effective manner, which is important for clinical treatments. Improved isolation and mass amplification of the hMSCs while ensuring their sternness and quantity will be an important step for clinical use. Enlarged suitable hMSCs are more clinically applicable for therapeutic transplants and may help people live longer and better.

引用

页码：399 / 406

页数：8

共 99 条

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A Comparative Study of Neural and Mesenchymal Stem Cell-Based Carriers for Oncolytic Adenovirus in a Model of Malignant Glioma [J].