Fluorogenic substrates for proteases based on intramolecular fluorescence energy transfer (IFETS)

被引:63
作者
Gershkovich, A [1 ]
Kholodovych, VV [1 ]
机构
[1] NATL ACAD SCI UKRAINE, INST BIOORGAN CHEM & PETROCHEM, DEPT BIOMED, KIEV, UKRAINE
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1996年 / 33卷 / 03期
关键词
intramolecular fluorescence energy transfer substrate; intramolecular quenched fluorescent substrate; protease; fluorescence;
D O I
10.1016/S0165-022X(96)00023-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A prospective class of intramolecular fluorescence energy transfer substrates (IFETS) is described. In contrast to the known chromogenic and fluorogenic substrates that are used widely in the scientific and medical research, IFETS allow to control the enzymatic cleavage at any point of the peptide chain and thus permit simultaneous studies of enzymes of different specificity. We discuss the main types of donor and acceptor, their advantages and drawbacks and the effectiveness of exited-state energy transfer between them. High sensitivity and selectivity of IFETS in the assay of proteinases was demonstrated. They prove to be a very useful and very promising instrument for enzymology and medicine.
引用
收藏
页码:135 / 162
页数:28
相关论文
共 78 条
  • [41] NEW SUBSTRATES FOR ENKEPHALINASE (NEUTRAL ENDOPEPTIDASE) BASED ON FLUORESCENCE ENERGY-TRANSFER
    MALFROY, B
    BURNIER, J
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 143 (01) : 58 - 66
  • [42] NOVEL FLUOROGENIC SUBSTRATES FOR ASSAYING RETROVIRAL PROTEASES BY RESONANCE ENERGY-TRANSFER
    MATAYOSHI, ED
    WANG, GT
    KRAFFT, GA
    ERICKSON, J
    [J]. SCIENCE, 1990, 247 (4945) : 954 - 958
  • [43] ANTHRANILAMIDE AND NITROTYROSINE AS A DONOR-ACCEPTOR PAIR IN INTERNALLY QUENCHED FLUORESCENT SUBSTRATES FOR ENDOPEPTIDASES - MULTICOLUMN PEPTIDE-SYNTHESIS OF ENZYME SUBSTRATES FOR SUBTILISIN CARLSBERG AND PEPSIN
    MELDAL, M
    BREDDAM, K
    [J]. ANALYTICAL BIOCHEMISTRY, 1991, 195 (01) : 141 - 147
  • [44] THE SPECIFICITY OF THE S(1)' SUBSITE OF CYSTEINE PROTEASES
    MENARD, R
    CARMONA, E
    PLOUFFE, C
    BROMME, D
    KONISHI, Y
    LEFEBVRE, J
    STORER, AC
    [J]. FEBS LETTERS, 1993, 328 (1-2) : 107 - 110
  • [45] CONTINUOUSLY RECORDING FLUORESCENT ASSAYS OPTIMIZED FOR 5 HUMAN MATRIX METALLOPROTEINASES
    NETZELARNETT, S
    MALLYA, SK
    NAGASE, H
    BIRKEDALHANSEN, H
    VANWART, HE
    [J]. ANALYTICAL BIOCHEMISTRY, 1991, 195 (01) : 86 - 92
  • [46] A FLUORESCENT OLIGOPEPTIDE ENERGY-TRANSFER ASSAY WITH BROAD APPLICATIONS FOR NEUTRAL PROTEASES
    NG, M
    AULD, DS
    [J]. ANALYTICAL BIOCHEMISTRY, 1989, 183 (01) : 50 - 56
  • [47] SUBSTRATE-SPECIFICITY OF THE HUMAN MATRIX METALLOPROTEINASE STROMELYSIN AND THE DEVELOPMENT OF CONTINUOUS FLUOROMETRIC ASSAYS
    NIEDZWIECKI, L
    TEAHAN, J
    HARRISON, RK
    STEIN, RL
    [J]. BIOCHEMISTRY, 1992, 31 (50) : 12618 - 12623
  • [48] 2-AMINOBENZOYL-PEPTIDE-2,4-DINITROANILINOETHYLAMIDES - FACILE FLUORESCENT DETECTION SYSTEM FOR SEQUENCE SPECIFIC PROTEASES
    NISHINO, N
    MAKINOSE, Y
    FUJIMOTO, T
    [J]. CHEMISTRY LETTERS, 1992, (01) : 77 - 80
  • [49] NISHINO N, 1980, J BIOL CHEM, V255, P3482
  • [50] INTRAMOLECULARLY QUENCHED FLUOROGENIC PEPTIDE-SUBSTRATES FOR HUMAN RENIN
    OLIVEIRA, MCF
    HIRATA, IY
    CHAGAS, JR
    BOSCHCOV, P
    GOMES, RAS
    FIGUEIREDO, AFS
    JULIANO, L
    [J]. ANALYTICAL BIOCHEMISTRY, 1992, 203 (01) : 39 - 46