PF15p is the Chlamydomonas homologue of the katanin p80 subunit and is required for assembly of flagellar central microtubules

被引:58
作者
Dymek, EE
Lefebvre, PA
Smith, EF
机构
[1] Dartmouth Coll, Dept Sci Biol, Hanover, NH 03755 USA
[2] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA
关键词
D O I
10.1128/EC.3.4.870-879.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Numerous studies have indicated that the central apparatus plays a significant role in regulating flagellar motility, yet little is known about how the central pair of microtubules or their associated projections assemble. Several Chlamydomonas mutants are defective in central apparatus assembly. For example, mutant pf15 cells have paralyzed flagella that completely lack the central pair of microtubules. We have cloned the wild-type PF15 gene and confirmed its identity by rescuing the motility and ultrastructural defects in two pf15 alleles, the original pf15a mutant and a mutant generated by insertional mutagenesis. Database searches using the 798-amino-acid polypeptide predicted from the complete coding sequence indicate that the PF15 gene encodes the Chlamydomonas homologue of the katanin p80 subunit. Katanin was originally identified as a heterodimeric protein with a microtubule-severing activity. These results reveal a novel role for the katanin p80 subunit in the assembly and/or stability of the central pair of flagellar microtubules.
引用
收藏
页码:870 / 879
页数:10
相关论文
共 69 条
[41]   Characterization of a Chlamydomonas insertional mutant that disrupts flagellar central pair microtubule-associated structures [J].
Mitchell, DR ;
Sale, WS .
JOURNAL OF CELL BIOLOGY, 1999, 144 (02) :293-304
[42]   Central-pair-linked regulation of microtubule sliding by calcium in flagellar axonernes [J].
Nakano, I ;
Kobayashi, T ;
Yoshimura, M ;
Shingyoji, C .
JOURNAL OF CELL SCIENCE, 2003, 116 (08) :1627-1636
[43]   THE CRY1 GENE IN CHLAMYDOMONAS-REINHARDTII - STRUCTURE AND USE AS A DOMINANT SELECTABLE MARKER FOR NUCLEAR TRANSFORMATION [J].
NELSON, JAE ;
SAVEREIDE, PB ;
LEFEBVRE, PA .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) :4011-4019
[44]   TRANSFORMATION OF CHLAMYDOMONAS-REINHARDTII [J].
NELSON, JAE ;
LEFEBVRE, PA .
METHODS IN CELL BIOLOGY, VOL 47, 1995, 47 :513-517
[45]   The 9+2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility [J].
Porter, ME ;
Sale, WS .
JOURNAL OF CELL BIOLOGY, 2000, 151 (05) :F37-F42
[46]   Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body [J].
Qin, HM ;
Diener, DR ;
Geimer, S ;
Cole, DG ;
Rosenbaum, JL .
JOURNAL OF CELL BIOLOGY, 2004, 164 (02) :255-266
[47]   SINGLE-GENE MUTATION OF CHLAMYDOMONAS REINHARDII AFFECTING MOTILITY - GENETIC + ELECTRON MICROSCOPE STUDY [J].
RANDALL, J ;
WARR, JR ;
MCVITTIE, A ;
HOPKINS, JM .
NATURE, 1964, 203 (494) :912-&
[48]  
Randall JT., 1967, DEVELOP BIOL S, V1, P43
[49]   The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility [J].
Rupp, G ;
O'Toole, E ;
Porter, ME .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (03) :739-751
[50]   NUTRITIONAL STUDIES WITH CHLAMYDOMANAS-REINHARDI [J].
SAGER, R ;
GRANICK, S .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1953, 56 (05) :831-838