Fluorescence lifetime and polarization-resolved imaging in cell biology

被引：161

作者：

Levitt, James A. ^{[1
]}

Matthews, Daniel R. ^{[2
]}

Ameer-Beg, Simon M. ^{[2
,3
]}

Suhling, Klaus ^{[1
]}

机构：

[1] Kings Coll London, Dept Phys, London WC2R 2LS, England

[2] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England

[3] Kings Coll London, Dimbleby Dept Canc Res, Div Canc Studies, London SE1 1UL, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2009年 / 20卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

RESONANCE ENERGY-TRANSFER; LIVING CELLS; LIVE CELLS; HOMO-FRET; MICROSCOPY; ANISOTROPY; MEMBRANE; PROTEINS; SPECTROSCOPY; DYNAMICS;

D O I：

10.1016/j.copbio.2009.01.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are complementary techniques that can be used to extract information about macromolecules from biological samples. Owing to the sensitivity of fluorescence to the physicochemical environment, and nanometer-scale interactions via Forster resonance energy transfer (FRET), FLIM has been implemented in many laboratories for numerous applications in the life sciences and beyond. This review seeks to provide a brief overview of some of the recent advances in the techniques and more pertinently their applications in cell and tissue imaging. The particular merits of polarization-resolved fluorescence measurements are highlighted, including the unique ability to elucidate the occurrence of homo-FRET.

引用

页码：28 / 36

页数：9

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