Label-free and reagentless aptamer-based sensors for small molecules

被引：276

作者：

Zayats, Maya

Huang, Ye

Gill, Ron

Ma, Chun-an

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

[2] Zhejiang Univ Technol, State Key Lab Green Chem Synth Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 42期

关键词：

D O I：

10.1021/ja0651456

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A label free, reagentless aptasensor for adenosine is developed on an ISFET device. The separation of an aptamer/nucleic acid duplex by adenosine leads to the aptamer/adenosine complex that alters the gate potential of the ISFET. The sensitivity limit of the device is 5 × 10-5 M. Also, the immobilization of the aptamer/nucleic acid duplex on an Au-electrode and the separation of the duplex by adenosine mono-phosphate (AMP) enable the electrochemical detection of adenosine by faradaic impedance spectroscopy. The separation of the aptamer/nucleic acid duplex by adenosine and the formation of the aptamer/adenosine complex results in a decrease in the interfacial electron-transfer resistance in the presence of [Fe(CN)6]3-/4- as redox active substrate. Copyright © 2006 American Chemical Society.

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页码：13666 / 13667

页数：2

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