Surface plasmon resonance (SPR) as a tool for antibody conjugate analysis

被引：38

作者：

Adamczyk, M ^{[1
]}

Mattingly, PG ^{[1
]}

Shreder, K ^{[1
]}

Yu, ZG ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Dept Chem D9NM, Abbott Pk, IL 60064 USA

来源：

BIOCONJUGATE CHEMISTRY | 1999年 / 10卷 / 06期

关键词：

D O I：

10.1021/bc990057e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Surface plasmon resonance (SPR) analysis was used to assess the immunoreactivity of anti-biotin (4) and anti-fluorescein (5) monoclonal antibody after conjugation with the N-hydroxysuccinimide ester of acridinium-9-carboxamide 1. Only minor changes in the apparent equilibrium dissociation constants of the antibody conjugates for their ligands resulted from the conjugation process. However, comparison of the initial binding rate of the conjugates with their ligands with those of the unmodified antibodies over a range of concentrations showed that the antibody conjugates were partially inactivated. The anti-fluorescein conjugates retained at least 90% of their immunoreactivity over the range of modification tested, while anti-biotin conjugates showed a progressive loss of reactivity with increased substitution by the label.

引用

页码：1032 / 1037

页数：6

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