Inhibition of Ca2+ channels in mouse spermatogenic cells by male antifertility compounds from Tripterygium wilfordii Hook. f.

被引：41

作者：

Bai, JP ^{[1
]}

Shi, YL ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Physiol, Key Lab Neurobiol, Shanghai, Peoples R China

来源：

CONTRACEPTION | 2002年 / 65卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Tripterygium wilfordii Hook. f; mouse; spermatogenic cell; Ca2+ channel; whole-cell recording; inhibition;

D O I：

10.1016/S0010-7824(02)00312-8

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The male antifertility effect of a water-chloroform extract (GTW) from the root xylem of Tripterygium wilfordii has attracted worldwide interest. In the present study, by using whole-cell recording, the effects of GTW and two isolated monomers from GTW, demethylzeylasteral and L-epicatechin. on the T-type Ca2+ channels in mouse spermatogenic cells were investigated. The results showed that each of them concentration-dependently and partially reversibly inhibited T-type Ca2+ current in the cells. The IC50 of GTW and demethylzeylasteral were approximate, while L-epicatechin inhibited the channels at a much higher concentration. The voltage dependence of the inhibitory effect and the changes in activation and inactivation time constants after application of these compounds were also examined. These data suggest that the inhibition of T-type Ca2+ Currents could be responsible for the antifertility activity of these compounds. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：441 / 445

页数：5

共 22 条

[11] Dual regulation of the T-type Ca2+ current by serum albumin and β-estradiol in mammalian spermatogenic cells [J].