High-Level Biosynthesis of the Anteiso-C17 Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity

被引：54

作者：

Fickers, Patrick ^{[1
]}

Guez, Jean-Sebastien ^{[2
]}

Damblon, Christian ^{[3
]}

Leclere, Valerie ^{[2
]}

Bechet, Max ^{[2
]}

Jacques, Philippe ^{[2
]}

Joris, Bernard

机构：

[1] Univ Liege, Inst Chim, Ctr Ingn Prot, Lab Physiol & Genet Bacterienne, B-4000 Liege, Belgium

[2] Univ Sci & Technol Lille, Lab Procedes Biol Genie Enzymat & Microbien, Polytech Lille, UPRES EA 1026, F-59655 Villeneuve Dascq, France

[3] Univ Liege, Inst Chim, B-4000 Liege, Belgium

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 13期

关键词：

SACCHAROMYCES-CEREVISIAE; GENETIC COMPETENCE; ITURIN-A; BIOCONTROL; MEMBRANE; OPERON; COMS;

D O I：

10.1128/AEM.00548-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

High-level production (880 mg liter(-1)) and isolation of the anteiso-C-17 isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.

引用

页码：4636 / 4640

页数：5

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