Molecular cloning and tissue-specific expression of mouse kidney 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

被引：8

作者：

Batra, RS ^{[1
]}

Brown, R ^{[1
]}

Brown, GK ^{[1
]}

Craig, IW ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,GENET LAB,OXFORD OX1 3QU,ENGLAND

来源：

FEBS LETTERS | 1996年 / 393卷 / 2-3期

关键词：

alternative splicing; fructose-2,6-bisphosphatase; fructose 2,6-bisphosphate; glycolysis; 6-phosphofructo-2-kinase; mouse kidney;

D O I：

10.1016/0014-5793(96)00878-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1932 bp cDNA clone encoding a novel isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) was isolated from a mouse kidney cDNA library, The sequence encodes 519 amino acids and, based on homology to rat heart genomic sequence, appears to be the product of alternative splicing from PFK-2/FBPase-2 gene B with an extended version of exon 15. Northern blot analysis indicated that this clone corresponds to an 8 kb mRNA expressed in multiple tissues, with the strongest signal in kidney, and detects several additional transcripts which may be alternatively spliced from gene B.

引用

页码：167 / 173

页数：7

共 33 条

[1] MOLECULAR-CLONING, SEQUENCE-ANALYSIS, AND EXPRESSION OF A HUMAN-LIVER CDNA CODING FOR FRUCTOSE-6-P,2-KINASE-FRUCTOSE-2,6-BISPHOSPHATASE [J].

ALGAIER, J ;

UYEDA, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 153 (01) :328-333

[2] EVOLUTION OF A BIFUNCTIONAL ENZYME - 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE [J].

BAZAN, JF ;

FLETTERICK, RJ ;

PILKIS, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :9642-9646

[3] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[4]

COLOSIA AD, 1988, J BIOL CHEM, V263, P18669

[5] CHARACTERIZATION OF DISTINCT MESSENGER-RNAS CODING FOR PUTATIVE ISOZYMES OF 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE [J].

CREPIN, KM ;

DARVILLE, MI ;

HUE, L ;

ROUSSEAU, GG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 183 (02) :433-440

[6] COMPLETE NUCLEOTIDE-SEQUENCE CODING FOR RAT-LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE DERIVED FROM A CDNA CLONE [J].

DARVILLE, MI ;

CREPIN, KM ;

VANDEKERCKHOVE, J ;

VANDAMME, J ;

OCTAVE, JN ;

RIDER, MH ;

MARCHAND, MJ ;

HUE, L ;

ROUSSEAU, GG .

FEBS LETTERS, 1987, 224 (02) :317-321

[7] 5' FLANKING SEQUENCE AND STRUCTURE OF A GENE ENCODING RAT 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE [J].

DARVILLE, MI ;

CREPIN, KM ;

HUE, L ;

ROUSSEAU, GG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6543-6547

[8] A RAT GENE ENCODING HEART 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE [J].

DARVILLE, MI ;

CHIKRI, M ;

LEBEAU, E ;

HUE, L ;

ROUSSEAU, GG .

FEBS LETTERS, 1991, 288 (1-2) :91-94

[9] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[10] CHARACTERIZATION OF A HEPATOMA MESSENGER-RNA TRANSCRIBED FROM A 3RD PROMOTER OF A 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE-ENCODING GENE AND CONTROLLED BY ETS ONCOGENE-RELATED PRODUCTS [J].

DUPRIEZ, VJ ;

DARVILLE, MI ;

ANTOINE, IV ;

GEGONNE, A ;

GHYSDAEL, J ;

ROUSSEAU, GG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8224-8228

← 1 2 3 4 →