Molecular cloning and tissue-specific expression of mouse kidney 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

被引：8

作者：

Batra, RS ^{[1
]}

Brown, R ^{[1
]}

Brown, GK ^{[1
]}

Craig, IW ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,GENET LAB,OXFORD OX1 3QU,ENGLAND

来源：

FEBS LETTERS | 1996年 / 393卷 / 2-3期

关键词：

alternative splicing; fructose-2,6-bisphosphatase; fructose 2,6-bisphosphate; glycolysis; 6-phosphofructo-2-kinase; mouse kidney;

D O I：

10.1016/0014-5793(96)00878-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1932 bp cDNA clone encoding a novel isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) was isolated from a mouse kidney cDNA library, The sequence encodes 519 amino acids and, based on homology to rat heart genomic sequence, appears to be the product of alternative splicing from PFK-2/FBPase-2 gene B with an extended version of exon 15. Northern blot analysis indicated that this clone corresponds to an 8 kb mRNA expressed in multiple tissues, with the strongest signal in kidney, and detects several additional transcripts which may be alternatively spliced from gene B.

引用

页码：167 / 173

页数：7

共 33 条

[31] CLONING AND EXPRESSION OF NOVEL ISOFORMS OF 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE FROM BOVINE HEART [J].

VIDAL, H ;

CREPIN, KM ;

RIDER, MH ;

HUE, L ;

ROUSSEAU, GG .

FEBS LETTERS, 1993, 330 (03) :329-333

[32] MOLECULAR-CLONING AND TISSUE-SPECIFIC EXPRESSION OF FRUCTOSE 6-PHOSPHATE,2-KINASE-FRUCTOSE 2,6-BISPHOSPHATASE OF RAT-BRAIN [J].

WATANABE, F ;

SAKAI, A ;

FURUYA, E ;

UYEDA, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 198 (01) :335-340

[33] LIVER-SPECIFIC DNASE I-HYPERSENSITIVE SITES AND DNA METHYLATION PATTERN IN THE PROMOTER REGION OF A 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE GENE [J].

ZIMMERMANN, PLN ;

ROUSSEAU, GG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 220 (01) :183-191

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