Two modes of fusion pore opening revealed by cell-attached recordings at a synapse

被引:156
作者
He, Liming [1 ]
Wu, Xin-Sheng [1 ]
Mohan, Raja [1 ]
Wu, Ling-Gang [1 ]
机构
[1] NINDS, Bethesda, MD 20892 USA
关键词
D O I
10.1038/nature05250
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Fusion of a vesicle with the cell membrane opens a pore that releases transmitter to the extracellular space(1-3). The pore can either dilate fully so that the vesicle collapses completely, or close rapidly to generate 'kiss-and-run' fusion(1,2,4-7). The size of the pore determines the release rate(2). At synapses, the size of the fusion pore is unclear, 'kiss-and-run' remains controversial(8-15), and the ability of 'kiss-and-run' fusion to generate rapid synaptic currents(16,17) is questionable(18). Here, by recording fusion pore kinetics during single vesicle fusion, we found both full collapse and 'kiss-and-run' fusion at calyx-type synapses. For full collapse, the initial fusion pore conductance (G(p)) was usually > 375 pS and increased rapidly at >= 299 pS ms(-1). 'Kiss-and-run' fusion was seen as a brief capacitance flicker (< 2 s) with G(p) > 288 pS for most flickers, but within 15 - 288 pS for the remaining flickers. Large G(p) (> 288 pS) might discharge transmitter rapidly and thereby cause rapid synaptic currents, whereas small Gp might generate slow and small synaptic currents. These results show that 'kiss-and-run' fusion occurs at synapses and that it can generate rapid postsynaptic currents, and suggest that various fusion pore sizes help to control the kinetics and amplitude of synaptic currents.
引用
收藏
页码:102 / 105
页数:4
相关论文
共 30 条
[21]   Modulation of glutamate mobility reveals the mechanism underlying slow-rising AMPAR EPSCs and the diffusion coefficient in the synaptic cleft [J].
Nielsen, TA ;
DiGregorio, DA ;
Silver, RA .
NEURON, 2004, 42 (05) :757-771
[22]   Postfusional control of quantal current shape [J].
Pawlu, C ;
DiAntonio, A ;
Heckmann, M .
NEURON, 2004, 42 (04) :607-618
[23]  
Sätzler K, 2002, J NEUROSCI, V22, P10567
[24]   Coordinated multivesicular release at a mammalian ribbon synapse [J].
Singer, JH ;
Lassová, L ;
Vardi, N ;
Diamond, JS .
NATURE NEUROSCIENCE, 2004, 7 (08) :826-833
[25]   PROPERTIES OF THE FUSION PORE THAT FORMS DURING EXOCYTOSIS OF A MAST-CELL SECRETORY VESICLE [J].
SPRUCE, AE ;
BRECKENRIDGE, LJ ;
LEE, AK ;
ALMERS, W .
NEURON, 1990, 4 (05) :643-654
[26]  
Stiles JR, 2001, SYNAPSES, P681
[27]   Miniature endplate current rise times <100 mu s from improved dual recordings can be modeled with passive acetylcholine diffusion from a synaptic vesicle [J].
Stiles, JR ;
VanHelden, D ;
Bartol, TM ;
Salpeter, EE ;
Salpeter, MM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :5747-5752
[28]   Single and multiple vesicle fusion induce different rates of endocytosis at a central synapse [J].
Sun, JY ;
Wu, XS ;
Wu, LG .
NATURE, 2002, 417 (6888) :555-559
[29]   Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical [J].
Wienisch, Martin ;
Klingauf, Jurgen .
NATURE NEUROSCIENCE, 2006, 9 (08) :1019-1027
[30]   Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse [J].
Yamashita, T ;
Hige, T ;
Takahashi, T .
SCIENCE, 2005, 307 (5706) :124-127