Effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondria dysfunction

被引：56

作者：

Chang, WH

Chen, CH

Gaul, RJ

Lin, CC

Tsai, CL

Tsai, K

Lu, FJ

机构：

[1] Natl Taiwan Univ, Coll Med, Grad Inst Biochem & Mol Biol, Taipei 100, Taiwan

[2] Kaohsiung Med Coll, Sch Pharm, Kaohsiung, Taiwan

[3] Natl Taiwan Univ, Coll Med, Dept Orthoped Surg, Taipei 10764, Taiwan

[4] Vet Gen Hosp, Oxidat Stress Clin Res Grp, Taipei, Taiwan

[5] Vet Gen Hosp, Intens Care Unit, Taipei, Taiwan

[6] Natl Yang Ming Univ, Sch Med, Taipei 112, Taiwan

来源：

PLANTA MEDICA | 2002年 / 68卷 / 04期

关键词：

Scutellaria baicalensis; Labiatae; baicalein; apoptosis; mitochondrial membrane potential; cytochrome c; Bcl-2;

D O I：

10.1055/s-2002-26760

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

The effects of baicalein on the human hepatoblastoma G2 (Hep G2) cell line were investigated in this study. By an SRB viability assay, we demonstrated that baicalein reduced the viability in a dose- and time-dependent manner. The apoptotic features such as chromatin condensation and DNA fragmentation were observed in the baicalein-treated cells. During the process of apoptosis, we noticed a sequential dissipation of mitochondrial membrane potential (DeltaPsi(m)) and an apparent redistribution of cytochrome c from the mitochondria to the cytosol in baicalein-treated cells. Furthermore, the mitochondrial Bcl-2 protein represented a dramatic change in response to baicalein treatment. Altogether, our data suggested that the effect of baicalein on apoptosis of the human Hep G2 cell line was induced by mitochondrial dysfunction and Bcl-2 regulation.

引用

页码：302 / 306

页数：5

共 20 条

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