Liquid chromatography/mass spectrometry in anabolic steroid analysis-optimization and comparison of three ionization techniques: electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization

被引：161

作者：

Leinonen, A

Kuuranne, T

Kostiainen, R ^{[1
]}

机构：

[1] Univ Helsinki, Dept Pharm, Div Pharmaceut Chem, FIN-00014 Helsinki, Finland

[2] United Labs Ltd, Doping Control Lab, Helsinki, Finland

[3] Univ Helsinki, Dept Pharm, Vikki Drug Discovery & Technol Ctr, FIN-00014 Helsinki, Finland

来源：

JOURNAL OF MASS SPECTROMETRY | 2002年 / 37卷 / 07期

关键词：

anabolic steroids; liquid chromatography/mass spectrometry; electrospray ionization; atmospheric pressure; chemical ionization; atmospheric pressure photoionization;

D O I：

10.1002/jms.328

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The applicability of liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the detection of the free anabolic steroid fraction in human urine was examined. Electrospray ionization (ESI), atmospheric pressure chemical ionization and atmospheric pressure photoionization methods were optimized regarding eluent composition, ion source parameters and fragmentation. The methods were compared with respect to specificity and detection limit. Although all methods proved suitable, LC/ESIMS/MS with a methanol-water gradient including 5 mm ammonium acetate and 0.01% acetic acid was found best for the purpose. Multiple reaction monitoring allowed the determination of steroids in urine at low nanogram per milliliter levels. LC/MS/MS exhibited high sensitivity and specificity for the detection of free steroids and may be a suitable technique for screening for the abuse of anabolic steroids in sports. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：693 / 698

页数：6

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