Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography

被引：80

作者：

Blesa, J ^{[1
]}

Berrada, H ^{[1
]}

Soriano, JM ^{[1
]}

Moltó, JC ^{[1
]}

Mañes, J ^{[1
]}

机构：

[1] Univ Valencia, Lab Food Chem & Toxicol, Fac Pharm, Burjassot 46100, Valencia, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1046卷 / 1-2期

关键词：

food analysis; cereals; ochratoxin A; mycotoxins;

D O I：

10.1016/j.chroma.2004.06.086

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method based on extraction with octylsilica (C-8) followed by liquid chromatography coupled with fluorescence detection (LC-FLD) was studied to determine ochratoxin A (OTA) from cereals and cereal products. Optimization of different parameters, such as type and amount of solid phase, type and volume of eluent and amount of sample were carried out. Recovery of OTA from rice samples spiked at 10 ng/g level was of 86% with relative standard deviation of 5%. The limits of detection and quantification of the proposed method were 0.25 and 0.75 ng/g, respectively. Furthermore, LC-FLD after of OTA methylation and liquid chromatography coupled to mass spectrometry with an electrospray interface were used for confirmation of OTA in all studied samples. The proposed method was applied to 62 samples of cereals and cereal products and the presence of OTA was found in seven samples. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：127 / 131

页数：5

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