Gene expression profiles in the cellular response to a multinuclear platinum complexe

被引：9

作者：

Gatti, L ^{[1
]}

Beretta, GL ^{[1
]}

Carenini, N ^{[1
]}

Corna, E ^{[1
]}

Zunino, F ^{[1
]}

Perego, P ^{[1
]}

机构：

[1] Ist Nazl Tumori, I-20133 Milan, Italy

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2004年 / 61卷 / 7-8期

关键词：

platinum compound; sensitivity; resistance; gene expression; cDNA array;

D O I：

10.1007/s00018-003-4024-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used cDNA arrays to monitor modulation of mRNA expression after exposure to a multinuclear platinum complex (BBR3464) in a human cervix squamous cell carcinoma cell line (A431) and in a cisplatin-resistant subline (A431/Pt) exhibiting collateral sensitivity to BBR3464. In parental A431cells, the drug induced at least twofold up-regulation of 15genes including cell cycle and growth regulators, tumor suppressors and signal transduction genes. In cisplatin-resistant A431/Pt cells, BBR3464increased the expression of 15genes such as apoptosis regulators and genes involved in the DNA damage response. Interestingly, BBR3464induced up-regulation of anti-metastatic factors together with down-regulation of several pro-metastatic factors. Cell cycle analysis indicated a marked G2arrest in treated A431cells, whereas an apoptotic response was documented in A431/Pt cells. These differential patterns of transcriptional profile in sensitive and resistant cells are consistent with a role for cell cycle regulation in the response to BBR3464.

引用

页码：973 / 981

页数：9

共 59 条

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