Cloth-based hybridization array system for detection of transgenic soy and corn by multiplex polymerase chain reaction

被引：15

作者：

Blais, BW ^{[1
]}

Phillippe, LM ^{[1
]}

Vary, N ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Ottawa Lab Carling, Ottawa, ON K1A 0C6, Canada

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 17期

关键词：

array; cloth; corn; hybridization; soy; transgenic;

D O I：

10.1023/A:1019894330731

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cloth-based hybridization array system (CHAS) was developed for detection of amplicons generated in a multiplex PCR targeting transgenic 35S and NOS sequences, as well as corn invertase and soy lectin genes. The CHAS provided confirmation of each amplicon on the basis of hybridization with specific capture probes, and enabled ease of discrimination of the multiplex PCR products by visualization of the amplicons on the array.

引用

页码：1407 / 1411

页数：5

共 3 条

[1] MACROPOROUS HYDROPHOBIC CLOTH (POLYMACRON) AS A SOLID-PHASE FOR NUCLEIC-ACID PROBE HYBRIDIZATIONS [J].

BLAIS, BW ;

PHILLIPPE, LM .

BIOTECHNOLOGY TECHNIQUES, 1995, 9 (05) :377-382

[2]

Lipp M, 1999, J AOAC INT, V82, P923

[3] Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods [J].

Vaïtilingom, M ;

Pijnenburg, H ;

Gendre, F ;

Brignon, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (12) :5261-5266

← 1 →