13C direct detected NMR increases the detectability of residual dipolar couplings

被引：47

作者：

Balayssac, Stephane

Bertini, Ivano

Luchinat, Claudio

Parigi, Giacomo

Piccioli, Mario

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem, I-50121 Florence, Italy

[3] Univ Florence, Dept Agr Biotechnol, I-50121 Florence, Italy

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 47期

关键词：

D O I：

10.1021/ja0645436

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

13C direct detection is becoming an increasingly efficient approach to identify signals of residues that escape detection in 1H detected experiments. Pulse sequences have been developed to obtain 1H partially recoupled experiments for the measurement of the 1JHαCα and 1JHN couplings with the same resolution available in conventional 1H detected experiments. A consistent set of backbone rdc obtained without any 1H-based experiment has been obtained and shown to be effective for protein solution structure determination. Copyright © 2006 American Chemical Society.

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页码：15042 / 15043

页数：2

相关论文

共 22 条

[1] Spin-state selection filters for the measurement of heteronuclear one-bond coupling constants [J].

Andersson, P ;

Weigelt, J ;

Otting, G .

JOURNAL OF BIOMOLECULAR NMR, 1998, 12 (03) :435-441

[2] Assignment strategy for fast relaxing signals:: complete aminoacid identification in thulium substituted Calbindin D9K [J].

Balayssac, S ;

Jiménez, B ;

Piccioli, M .

JOURNAL OF BIOMOLECULAR NMR, 2006, 34 (02) :63-73

[3] Backbone-only protein solution structures with a combination of classical and paramagnetism-based constraints: A method that can be scaled to large molecules [J].

Barbieri, R ;

Luchinat, C ;

Parigi, G .

CHEMPHYSCHEM, 2004, 5 (06) :797-806

[4] 13C-detected protonless NMR spectroscopy of proteins in solution [J].

Bermel, W ;

Bertini, I ;

Felli, IC ;

Piccioli, M ;

Pierattelli, R .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2006, 48 (01) :25-45

[5] Protonless NMR experiments for sequence-specific assignment of backbone nuclei in unfolded proteins [J].

Bermel, W ;

Bertini, I ;

Felli, IC ;

Lee, YM ;

Luchinat, C ;

Pierattelli, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (12) :3918-3919

[6] Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins [J].

Bermel, W ;

Bertini, I ;

Felli, IC ;

Kümmerle, R ;

Pierattelli, R .

JOURNAL OF MAGNETIC RESONANCE, 2006, 178 (01) :56-64

[7]

Bertini I, 2001, CHEMBIOCHEM, V2, P550, DOI 10.1002/1439-7633(20010803)2:7/8<550::AID-CBIC550>3.3.CO

[8]

2-K

[9] Mapping protein-protein interaction by 13C′-detected heteronuclear NMR spectroscopy [J].

Bertini, Ivano ;

Felli, Isabella C. ;

Gonnelli, Leonardo ;

Pierattelli, Roberta ;

Spyranti, Zinovia ;

Spyroulias, Georgios A. .

JOURNAL OF BIOMOLECULAR NMR, 2006, 36 (02) :111-122

[10] Direct-detected 13C NMR to investigate the iron(III) hemophore HasA [J].

Caillet-Saguy, C ;

Delepierre, M ;

Lecroisey, A ;

Bertini, I ;

Piccioli, M ;

Turano, P .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (01) :150-158