Multiplexed Detection of Bacteria and Toxins Using a Microflow Cytometer

被引：83

作者：

Kim, Jason S. ^{[1
]}

Anderson, George P. ^{[1
]}

Erickson, Jeffrey S. ^{[1
]}

Golden, Joel P. ^{[1
]}

Nasir, Mansoor ^{[1
]}

Ligler, Frances S. ^{[1
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 13期

关键词：

FLOW CYTOMETER; ARRAY IMMUNOSENSOR; SYSTEM;

D O I：

10.1021/ac9005827

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A microfabricated flow cytometer was used to demonstrate multiplexed detection of bacteria and toxins using fluorescent coded microspheres. Antibody-coated microspheres bound biothreat targets in a sandwich immunoassay format. The microfluidic cytometer focused the microspheres in three dimensions within the laser interrogation region using passive groove structures to surround the sample stream with sheath fluid. Optical analysis at four different wavelengths identified the coded microspheres and quantified target bound by the presence of phycoerythrin tracer. The multiplexed assays in the microflow cytometer had performance approaching that of a commercial benchtop flow cytometer. The respective limits of detection for bacteria (Escherichia coli, Listeria, and Salmonella) were found to be 10(3), 10(5), and 10(4) cfu/mL for the microflow cytometer and 10(3), 10(6), and 10(5) cfu/mL for the commercial system. limits of detection for the toxins (cholera toxin, staphylococcal enterotoxin B, and ricin) were 1.6, 0.064, and 1.6 ng/mL for the microflow cytometer and 1.6, 0.064, and 8.0 ng/mL for the commercial system.

引用

页码：5426 / 5432

页数：7

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