The good, the bad, and the tiny: a review of microflow cytometry

被引：185

作者：

Ateya, Daniel A. ^{[1
]}

Erickson, Jeffrey S. ^{[1
]}

Howell, Peter B., Jr. ^{[1
]}

Hilliard, Lisa R. ^{[1
]}

Golden, Joel P. ^{[1
]}

Ligler, Frances S. ^{[1
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 391卷 / 05期

关键词：

flow cytometry; microfluidics; fluid focusing; integrated optics; cell sorter;

D O I：

10.1007/s00216-007-1827-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in microflow cytometry have concentrated on advancing technology in four main areas: (1) focusing the particles to be analyzed in the microfluidic channel, (2) miniaturization of the fluid-handling components, (3) miniaturization of the optics, and (4) integration and applications development. Strategies for focusing particles in a narrow path as they pass through the detection region include the use of focusing fluids, nozzles, and dielectrophoresis. Strategies for optics range from the use of microscope objectives to polymer waveguides or optical fibers embedded on-chip. While most investigators use off-chip fluidic control, there are a few examples of integrated valves and pumps. To date, demonstrations of applications are primarily used to establish that the microflow systems provide data of the same quality as laboratory systems, but new capabilities-such as automated sample staining-are beginning to emerge. Each of these four areas is discussed in detail in terms of the progress of development, the continuing limitations, and potential future directions for microflow cytometers.

引用

页码：1485 / 1498

页数：14

共 95 条

[1] Microfluidic integration on detector arrays for absorption and fluorescence micro-spectrometers [J].

Adams, ML ;

Enzelberger, M ;

Quake, S ;

Scherer, A .

SENSORS AND ACTUATORS A-PHYSICAL, 2003, 104 (01) :25-31

[2] Steady flow generation in microcirculatory systems [J].

Atencia, J ;

Beebe, DJ .

LAB ON A CHIP, 2006, 6 (04) :567-574

[3] Lab-on-a-chip with integrated optical transducers [J].

Balslev, S ;

Jorgensen, AM ;

Bilenberg, B ;

Mogensen, KB ;

Snakenborg, D ;

Geschke, O ;

Kutter, JP ;

Kristensen, A .

LAB ON A CHIP, 2006, 6 (02) :213-217

[4] Expansion channel for microchip flow cytometers [J].

Bang, Hyunwoo ;

Yun, Hoyoung ;

Lee, Won Gu ;

Park, Junha ;

Lee, Jooruno ;

Chung, Seok ;

Cho, Keunchang ;

Chung, Chanil ;

Han, Dong-Chul ;

Chang, Jun Kenn .

LAB ON A CHIP, 2006, 6 (10) :1381-1383

[5] Development and characterization of an integrated silicon micro flow cytometer [J].

Bernini, R. ;

De Nuccio, E. ;

Brescia, F. ;

Minardo, A. ;

Zeni, L. ;

Sarro, P. M. ;

Palumbo, R. ;

Scarfi, M. R. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 386 (05) :1267-1272

[6] Modular concept of a laboratory on a chip for chemical and biochemical analysis [J].

Blankenstein, G ;

Larsen, UD .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (3-4) :427-438

[7]

Blankenstein G, 1996, CYTOMETRY, V25, P200, DOI 10.1002/(SICI)1097-0320(19961001)25:2<200::AID-CYTO9>3.0.CO

[8]

2-K

[9] An integrated nanoliter DNA analysis device [J].

Burns, MA ;

Johnson, BN ;

Brahmasandra, SN ;

Handique, K ;

Webster, JR ;

Krishnan, M ;

Sammarco, TS ;

Man, PM ;

Jones, D ;

Heldsinger, D ;

Mastrangelo, CH ;

Burke, DT .

SCIENCE, 1998, 282 (5388) :484-487

[10] PDMS 2D optical lens integrated with microfluidic channels: principle and characterization [J].

Camou, S ;

Fujita, H ;

Fujii, T .

LAB ON A CHIP, 2003, 3 (01) :40-45

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