Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies

被引：134

作者：

Hilliard, LR

Zhao, XJ

Tan, WH ^{[1
]}

机构：

[1] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA

[2] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 470卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

covalent immobilization; oligonucleotides; silica nanoparticles; disulfide-coupling; thiol/disulfide exchange reaction;

D O I：

10.1016/S0003-2670(02)00538-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the development of oligonucleotide-functionalized nanoparticles. We used disulfide-coupling chemistry for the immobilization of oligonucleotides onto silica nanoparticles and subsequently demonstrated the properties of the resulting DNA nanoparticles. Factors influencing the immobilization and hybridization processes were examined and optimized. The oligonucleotide-modified silica nanoparticles provide an efficient substrate for hybridization and can be used in the development of DNA biosensors and biochips. (C) 2002 Elsevier Science B.V. All rights reserved.

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收藏

页码：51 / 56

页数：6

相关论文

共 20 条

[1] Hybridization of DNA targets to glass-tethered oligonucleotide probes [J].

Beattie, WG ;

Meng, L ;

Turner, SL ;

Varma, RS ;

Dao, DD ;

Beattie, KL .

MOLECULAR BIOTECHNOLOGY, 1995, 4 (03) :213-225

[2] INTRODUCTION OF 5'-TERMINAL FUNCTIONAL-GROUPS INTO SYNTHETIC OLIGONUCLEOTIDES FOR SELECTIVE IMMOBILIZATION [J].

BISCHOFF, R ;

COULL, JM ;

REGNIER, FE .

ANALYTICAL BIOCHEMISTRY, 1987, 164 (02) :336-344

[3] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[4] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[5] Covalent attachment of synthetic DNA to self-assembled monolayer films [J].

Chrisey, LA ;

Lee, GU ;

OFerrall, CE .

NUCLEIC ACIDS RESEARCH, 1996, 24 (15) :3031-3039

[6] LIGATION OF OLIGONUCLEOTIDES TO NUCLEIC-ACIDS OR PROTEINS VIA DISULFIDE BONDS [J].

CHU, BCF ;

ORGEL, LE .

NUCLEIC ACIDS RESEARCH, 1988, 16 (09) :3671-3691

[7] IMMOBILIZATION OF POLYNUCLEOTIDES ON MAGNETIC PARTICLES - FACTORS INFLUENCING HYBRIDIZATION EFFICIENCY [J].

DAY, PJR ;

FLORA, PS ;

FOX, JE ;

WALKER, MR .

BIOCHEMICAL JOURNAL, 1991, 278 :735-740

[8] DIRECT FLUORESCENCE ANALYSIS OF GENETIC POLYMORPHISMS BY HYBRIDIZATION WITH OLIGONUCLEOTIDE ARRAYS ON GLASS SUPPORTS [J].

GUO, Z ;

GUILFOYLE, RA ;

THIEL, AJ ;

WANG, RF ;

SMITH, LM .

NUCLEIC ACIDS RESEARCH, 1994, 22 (24) :5456-5465

[9] Covalent immobilization of single-stranded DNA onto optical fibers using various linkers [J].

Henke, L ;

Piunno, PAE ;

McClure, AC ;

Krull, UJ .

ANALYTICA CHIMICA ACTA, 1997, 344 (03) :201-213

[10] Covalent attachment of hybridizable oligonucleotides to glass supports [J].

Joos, B ;

Kuster, H ;

Cone, R .

ANALYTICAL BIOCHEMISTRY, 1997, 247 (01) :96-101