NTA Directed Protein Nanopatterning on DNA Origami Nanoconstructs

被引：145

作者：

Shen, Wanqiu ^{[1
]}

Zhong, Hong ^{[1
]}

Neff, David ^{[1
]}

Norton, Michael L. ^{[1
]}

机构：

[1] Marshall Univ, Dept Chem, Huntington, WV 25755 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 19期

基金：

美国国家科学基金会;

关键词：

GREEN FLUORESCENT PROTEIN; NANOARRAYS; ARRAYS; BINDING;

D O I：

10.1021/ja901407j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Precisely patterning proteins and other molecules at the nanoscale is crucial to future biosensing and optoelectronic applications. One- and two-dimensional DNA nanoconstructs have proven to be useful scaffolds for nanopatterning. This paper demonstrates the application of nitrilotriacetic acid (NTA) forming chelate complexes to localize histidine (His) tagged proteins via Ni-2 center dot ions onto DNA based structures. Particularly, enhanced green fluorescent protein (EGFP) was directed to specific surface locations on a designed DNA Origami nanoconstruct, and the resulting EGFP nanopattern was visualized using atomic force microscopy (AFM).

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页码：6660 / +

页数：3

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