An application of high-speed counter-current chromatography coupled with electrospray ionization mass spectrometry for separation and online identification of coumarins from Peucedanum praeruptorum Dunn

被引：44

作者：

Hou, Zhiguo ^{[1
]}

Xu, Deran ^{[1
]}

Yao, Shun ^{[1
]}

Luo, Jianguang ^{[1
]}

Kong, Lingyi ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 24期

关键词：

prep-HSCCC/ESI-MSn; Coumarins; Peucedanum praeruptorum Dunn; DRUG QIAN-HU; PREPARATIVE ISOLATION; CHEMICAL-CONSTITUENTS; PURIFICATION; ROOT;

D O I：

10.1016/j.jchromb.2009.06.042

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new and systematic application for separation and online identification of coumarins from Peucedanum praeruptorum Dunn by preparative high-speed counter-current chromatography coupled with electrospray ionization multi-stage mass spectrometry (prep-HSCCC/ESI-MSn) was established. The procedure of separation was guided by the chromatogram of ion current. The structures of acquisitions were deduced by MS information. The hyphenation between prep-HSCCC/ESI-MSn was designed to keep the split ratio from 1:20 to 1:200 exactly. Seven compounds were obtained and two new compounds were detected. It was proved that prep-HSCCC/ESI-MSn was an effective method for sensitive detection, rapid identification and separation of natural products. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2571 / 2578

页数：8

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