Self-assembly of supramolecular aptamer structures for optical or electrochemical sensing

被引：102

作者：

Freeman, Ronit ^{[1
]}

Li, Yang ^{[1
]}

Tel-Vered, Ran ^{[1
]}

Sharon, Etery ^{[1
]}

Elbaz, Johann ^{[1
]}

Willner, Itamar ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel

来源：

ANALYST | 2009年 / 134卷 / 04期

基金：

以色列科学基金会;

关键词：

SURFACE-PLASMON RESONANCE; CATALYTIC LABELS; QUANTUM DOTS; BIOSENSOR; SENSOR; DNA; NANOPARTICLES; BEACONS; COCAINE;

D O I：

10.1039/b822836c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The self-assembly of labeled aptamer sub-units in the presence of their substrates provides a method for the optical (fluorescence) or electrochemical detection of the substrate. One of the sub-units is linked to CdSe/ZnS quantum dots (QDs), and the self-assembly of the dye-functionalized second sub-unit with the modified QDs, in the presence of cocaine, stimulates fluorescence resonance energy transfer (FRET). This enables the detection of cocaine with a detection limit corresponding to 1 x 10(-6) M. Alternatively, the aptamer fragments are modified with pyrene units. The formation of a supramolecular aptamer-substrate complex allosterically stabilizes the formation of excimer supramolecular structure, and its characteristic emission is observed. In addition, the thiolated aptamer sub-unit is assembled on an Au electrode. The Methylene Blue-labeled sub-unit binds to the surface-confined fragment in the presence of cocaine. The amperometric response of the system allows the detection of cocaine with a detection limit of 1 x 10(-5) M. The approach is generic and can be applied to other substrates, e. g. adenosine triphosphate.

引用

页码：653 / 656

页数：4

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