Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy

被引：222

作者：

Bennett, TN

Paguio, M

Gligorijevic, B

Seudieu, C

Kosar, AD

Davidson, E

Roepe, PD

机构：

[1] Georgetown Univ, Dept Chem, Washington, DC 20057 USA

[2] Georgetown Univ, Dept Biochem & Mol Biol, Washington, DC 20057 USA

[3] Georgetown Univ, Lombardi Canc Ctr, Washington, DC 20057 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 05期

关键词：

D O I：

10.1128/AAC.48.5.1807-1810.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [H-3]hypoxanthine and [H-3]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.

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页码：1807 / 1810

页数：4

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