SR Protein Family Members Display Diverse Activities in the Formation of Nascent and Mature mRNPs In Vivo

被引：110

作者：

Sapra, Aparna K. ^{[1
]}

Aenkoe, Minna-Liisa ^{[1
]}

Grishina, Inna ^{[1
]}

Lorenz, Mike ^{[1
]}

Pabis, Marta ^{[1
]}

Poser, Ina ^{[1
]}

Rollins, Jarod ^{[1
]}

Weiland, Eva-Maria ^{[1
]}

Neugebauer, Karla M. ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

来源：

MOLECULAR CELL | 2009年 / 34卷 / 02期

关键词：

PRE-MESSENGER-RNA; SPLICING FACTOR; POLYMERASE-II; PROTEOMIC ANALYSIS; TERMINAL DOMAIN; TRANSCRIPTION; SPLICEOSOME; NUCLEAR; EXON; RECRUITMENT;

D O I：

10.1016/j.molcel.2009.02.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SIR proteins are a family of pre-mRNA splicing factors with additional roles in gene regulation. To investigate individual family members in vivo, we generated a comprehensive panel of stable cell lines expressing GFP-tagged SR proteins under endogenous promoter control. Recruitment of SIR proteins to nascent FOS RNA was transcription dependent and RNase sensitive, with unique patterns of accumulation along the gene specified by the RNA recognition motifs (RRMs). In addition, all SIR protein interactions with Pol II were RNA dependent, indicating that SR proteins are not preassembled with Pol II. SR protein interactions with RNA were confirmed in situ by FRET/FLIM. Interestingly, SC35-GFP also exhibited FRET with DNA and failed to associate with cytoplasmic m RNAs, whereas all other SR proteins underwent nucleocytoplasmic shuttling and associated with specific nuclear and cytoplasmic mRNAs. Because different constellations of SIR proteins bound nascent, nuclear, and cytoplasmic mRNAs, mRNP remodeling must occur throughout an mRNA's lifetime.

引用

页码：179 / 190

页数：12

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