Characterization of the second form of NADH-cytochrome b5 reductase gene from arachidonic acid-producing fungus Mortierella alpina 1S-4

被引：13

作者：

Certik, M ^{[1
]}

Sakuradani, E ^{[1
]}

Kobayashi, M ^{[1
]}

Shimizu, S ^{[1
]}

机构：

[1] Kyoto Univ, Grad Shc Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 88卷 / 06期

关键词：

cytochrome b(5) reductase; cloning; Mortierella fungus;

D O I：

10.1016/S1389-1723(00)87098-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The second type of cytochrome b(5) reductase (Cb5R-II) gene was characterized in the arachidonic acid-producing fungus Mortierella alpina 1S-4. Its cDNA (897 bp) and predicted amino acid (298 as) sequences show more than 70% similarity to the previously isolated first type of Cb5R. Highly conserved exon-intron organization suggests that the two genes evolved from the duplication of a common ancestral gene. Cb5R-II has a flavin-binding domain at its highly hydrophobic N-terminal and an NADH-binding domain at the C-terminal. In comparison with Cb5R genes from other sources, high homology (46-54%) was found for yeast and plant genes. Phylogenetic analysis revealed that microbial and plant Cb5R genes represent a gene family evolved from one prototype and are different from mammalian Cb5R genes.

引用

页码：667 / 671

页数：5

共 23 条

[1] Desaturase-defective fungal mutants: useful tools for the regulation and overproduction of polyunsaturated fatty acids [J].

Certik, M ;

Sakuradani, E ;

Shimizu, S .

TRENDS IN BIOTECHNOLOGY, 1998, 16 (12) :500-505

[2] Biosynthesis and regulation of microbial polyunsaturated fatty acid production [J].

Certik, M ;

Shimizu, S .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (01) :1-14

[3]

Certik M, 1999, AGRO FOOD IND HI TEC, V10, P26

[4] ISOLATION AND COMPLETE SEQUENCE OF CBR, A GENE ENCODING A PUTATIVE CYTOCHROME-B REDUCTASE IN SACCHAROMYCES-CEREVISIAE [J].

CSUKAI, M ;

MURRAY, M ;

ORR, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (1-2) :441-448

[5] Microsomal electron transfer in higher plants:: Cloning and heterologous expression of NADH-cytochrome b5 reductase from arabidopsis [J].

Fukuchi-Mizutani, M ;

Mizutani, M ;

Tanaka, Y ;

Kusumi, T ;

Ohta, D .

PLANT PHYSIOLOGY, 1999, 119 (01) :353-361

[6] INCOMPLETE ARREST IN THE OUTER-MEMBRANE SORTS NADH-CYTOCHROME-B(5) REDUCTASE TO 2 DIFFERENT SUBMITOCHONDRIAL COMPARTMENTS [J].

HAHNE, K ;

HAUCKE, V ;

RAMAGE, L ;

SCHATZ, G .

CELL, 1994, 79 (05) :829-839

[7] Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain [J].

Kimura, S ;

Emi, Y ;

Ikushiro, S ;

Iyanagi, T .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1999, 1430 (02) :290-301

[8] Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in bakers' yeast and canola [J].

Knutzon, DS ;

Thurmond, JM ;

Huang, YS ;

Chaudhary, S ;

Bobik, EG ;

Chan, GM ;

Kirchner, SJ ;

Mukerji, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (45) :29360-29366

[9] Genetic analysis of cytochrome b5 from arachidonic acid-producing fungus, Mortierella alpina 1S-4:: Cloning, RNA editing and expression of the gene in Escherichia coli, and purification and characterization of the gene product [J].

Kobayashi, M ;

Sakuradani, E ;

Shimizu, S .

JOURNAL OF BIOCHEMISTRY, 1999, 125 (06) :1094-1103

[10] AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS [J].

KOZAK, M .

NUCLEIC ACIDS RESEARCH, 1987, 15 (20) :8125-8148

← 1 2 3 →