Functional Characterisation and Permeation Studies of Lyophilised Thiolated Chitosan Xerogels for Buccal Delivery of Insulin

被引:28
作者
Boateng, Joshua S. [1 ]
Mitchell, John C. [1 ]
Pawar, Harshavardhan [1 ]
Ayensu, Isaac [1 ]
机构
[1] Univ Greenwich Medway, Fac Sci & Engn, Dept Pharmaceut Chem & Environm Sci, Chatham ME4 4TB, Kent, England
关键词
Buccal mucosa; chitosan; enzyme inhibitor; insulin; permeation enhancement; xerogel; IN-VITRO EVALUATION; DRUG-DELIVERY; FILMS; VIVO; SYSTEMS; WAFERS; PERMEABILITY; MEMBRANE; POLYMERS; THIOMERS;
D O I
10.2174/0929866521666140805124403
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stable and mucoadhesive, lyophilised, thiolated chitosan xerogels, loaded with insulin for buccal mucosa delivery, in place of the currently used parenteral route have been developed. The xerogels were backed with impervious ethyl-cellulose laminate to ensure unidirectional release and also loaded with enzyme inhibitor to enhance insulin permeability across the buccal mucosa. Characterisation of xerogels using (HNMR)-H-1 confirmed the degree of deacetylation of the synthesised thiolated chitosan. The amount of thiol groups immobilised on the modified chitosan was quantified by Ellman's reaction and molecular weight monitored by gel permeation chromatography. The stability of the secondary structure of insulin was examined by attenuated total reflectance Fourier transform infra-red spectroscopy and circular dichroism. In vitro and ex vivo permeation studies were undertaken by using EpiOral (TM) and sheep buccal membrane respectively. Insulin released from thiolated chitosan xerogels, loaded with aprotinin (enzyme inhibitor and permeation enhancer) showed a 1.7-fold increase in permeation through EpiOral (TM) buccal tissue construct compared to the pure drug. However, permeation was decreased for xerogels containing the enzyme inhibitor glutathione. Further, aprotinin containing xerogels enhanced insulin permeation through sheep buccal membrane and demonstrated good linear correlation with the permeation data from the EpiOral (TM) study. The results show the potential application of lyophilised thiolated chitosan xerogels containing aprotinin with improved mucoadhesion, penetration enhancing and enzyme inhibition characteristics for buccal mucosa delivery of macromolecules such as insulin.
引用
收藏
页码:1163 / 1175
页数:13
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