Activation of Na+ channels in GH(3) cells and human pituitary adenoma cells by PACAP

被引：15

作者：

Koshimura, K ^{[1
]}

Murakami, Y ^{[1
]}

Mitsushima, M ^{[1
]}

Hori, T ^{[1
]}

Kato, Y ^{[1
]}

机构：

[1] TOTTORI UNIV,FAC MED,DEPT NEUROSURG,YONAGO,TOTTORI 683,JAPAN

来源：

PEPTIDES | 1997年 / 18卷 / 06期

关键词：

PACAP; Na+ channel; tetrodotoxin; cAMP; protein kinase A; Ca2+ channel; GH(3) cells; human pituitary adenoma; patch clamp;

D O I：

10.1016/S0196-9781(97)00019-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of pituitary adenylate cyclase activating polypeptide (PACAP) on ion channels were examined in GH(3) cells and human pituitary adenoma cells. In GH(3) cells, PACAP-38 (10-9 M) reversibly activated tetrodotoxin-sensitive Na+ channels but had little effect on nicardipine-sensitive Ca2+ channels. PACAP-induced increase in Na+ currents was inhibited by PACAP(6-38), a specific PACAP receptor antagonist, and Rp-cAMPs, an inhibitor for protein kinase A, and mimicked by 8-bromo-cAMP. In human pituitary adenoma cells, PACAP also activated tetrodotoxin-sensitive Na+ channels and growth hormone secretion. These results suggest the possibility that PACAP can activate voltage-gated Na+ channels via adenylate cyclase-protein kinase A pathway in the pituitary. (C) 1997 Elsevier Science Inc.

引用

页码：877 / 883

页数：7

共 31 条

[21] PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) STIMULATES GROWTH-HORMONE RELEASE FROM GH(3) CELLS THROUGH TYPE-II PACAP RECEPTOR [J].

MURAKAMI, Y ;

KOSHIMURA, K ;

YAMAUCHI, K ;

NISHIKI, M ;

TANAKA, J ;

FURUYA, H ;

MIYAKE, T ;

KATO, Y .

REGULATORY PEPTIDES, 1995, 56 (01) :35-40

[22]

PROPATOMUSSAFIRI R, 1992, J ENDOCRINOL, V132, P107, DOI 10.1677/joe.0.1320107

[23]

RAWLINGS SR, 1994, J BIOL CHEM, V269, P5680

[24] PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE REGULATES CYTOSOLIC CA2+ IN RAT GONADOTROPES AND SOMATOTROPES THROUGH DIFFERENT INTRACELLULAR MECHANISMS [J].

RAWLINGS, SR ;

CANNY, BJ ;

LEONG, DA .

ENDOCRINOLOGY, 1993, 132 (04) :1447-1452

[25] Pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP/vasoactive intestinal polypeptide receptors: Actions on the anterior pituitary gland [J].

Rawlings, SR ;

Hezareh, M .

ENDOCRINE REVIEWS, 1996, 17 (01) :4-29

[26] EFFECTS OF PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE IN THE PITUITARY - ACTIVATION OF 2 SIGNAL-TRANSDUCTION PATHWAYS IN THE GONADOTROPE-DERIVED ALPHA-T3-1 CELL-LINE [J].

SCHOMERUS, E ;

POCH, A ;

BUNTING, R ;

MASON, WT ;

MCARDLE, CA .

ENDOCRINOLOGY, 1994, 134 (01) :315-323

[27] Stimulating effect of 6R-tetrahydrobiopterin on CA(2+) channels in neurons of rat dorsal motor nucleus of the vagus [J].

Shiraki, T ;

Koshimura, K ;

Kobayashi, S ;

Miwa, S ;

Masaki, T ;

Watanabe, Y ;

Murakami, Y ;

Kato, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (01) :181-185

[28]

STOJILKOVIC SS, 1988, J BIOL CHEM, V263, P13054

[29] 2 TYPES OF CALCIUM CHANNELS AND HORMONE-RELEASE IN HUMAN PITUITARY-TUMOR CELLS [J].

YAMASHITA, N ;

MATSUNAGA, H ;

SHIBUYA, N ;

TERAMOTO, A ;

TAKAKURA, K ;

OGATA, E .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (02) :E137-E145

[30] Involvement of pituitary adenylate cyclase-activating polypeptide in growth hormone secretion induced by serotoninergic mechanisms in the rat [J].

Yamauchi, K ;

Murakami, Y ;

Koshimura, K ;

Nishiki, M ;

Tanaka, J ;

Kato, Y .

ENDOCRINOLOGY, 1996, 137 (05) :1693-1697

← 1 2 3 4 →