Activation of Na+ channels in GH(3) cells and human pituitary adenoma cells by PACAP

被引：15

作者：

Koshimura, K ^{[1
]}

Murakami, Y ^{[1
]}

Mitsushima, M ^{[1
]}

Hori, T ^{[1
]}

Kato, Y ^{[1
]}

机构：

[1] TOTTORI UNIV,FAC MED,DEPT NEUROSURG,YONAGO,TOTTORI 683,JAPAN

来源：

PEPTIDES | 1997年 / 18卷 / 06期

关键词：

PACAP; Na+ channel; tetrodotoxin; cAMP; protein kinase A; Ca2+ channel; GH(3) cells; human pituitary adenoma; patch clamp;

D O I：

10.1016/S0196-9781(97)00019-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of pituitary adenylate cyclase activating polypeptide (PACAP) on ion channels were examined in GH(3) cells and human pituitary adenoma cells. In GH(3) cells, PACAP-38 (10-9 M) reversibly activated tetrodotoxin-sensitive Na+ channels but had little effect on nicardipine-sensitive Ca2+ channels. PACAP-induced increase in Na+ currents was inhibited by PACAP(6-38), a specific PACAP receptor antagonist, and Rp-cAMPs, an inhibitor for protein kinase A, and mimicked by 8-bromo-cAMP. In human pituitary adenoma cells, PACAP also activated tetrodotoxin-sensitive Na+ channels and growth hormone secretion. These results suggest the possibility that PACAP can activate voltage-gated Na+ channels via adenylate cyclase-protein kinase A pathway in the pituitary. (C) 1997 Elsevier Science Inc.

引用

页码：877 / 883

页数：7