Agrobacterium tumefaciens-mediated transformation of Paracoccidioides brasiliensis

被引:18
作者
Leal, CV
Montes, BA
Mesa, AC
Rua, AL
Corredor, M
Restrepo, A
McEwen, JG
机构
[1] CIB, Cellular Biol & Immunogenet Grp, Medellin, Colombia
[2] CIB, Med & Expt Mycol Grp, Medellin, Colombia
[3] CIB, Grp Genet & Biochem Microorganisms, Medellin, Colombia
[4] Univ Pamplona, Pamplona, Spain
[5] Univ Pontificia Bolivariana, Postgrad Studies Ctr, Medellin, Colombia
[6] Univ Antioquia, Dept Microbiol & Parasitol, Medellin, Colombia
[7] Univ Antioquia, Sch Bacteriol & Clin Lab, Medellin, Colombia
关键词
A; tumefaciens; P; brasiliensis; transformation;
D O I
10.1080/13693780410001712007
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, an important mycosis endemic to Latin America. As the tools to study gene function in P. brasiliensis are only in the early stage of development, there is presently no system that allows for both the delivery and integration of exogenous nucleic acids into its genome. We report in this paper the transformation of the yeast phase of P. brasiliensis (ATCC-60855) with Agrobacterium tumefaciens (GV3101) carrying the vector pAD1625. The microorganisms were co-cultivated for 2 days and then incubated for 10 days at 35degreesC on selective media. PCR and dot-blot targeted at a fragment of 222 by from the hph (hygromycin phosphotransferase) gene which confers Hyg(r) confirmed the transformation of P. brasiliensis.
引用
收藏
页码:391 / 395
页数:5
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