Overview of Electrochemical DNA Biosensors: New Approaches to Detect the Expression of Life

被引：94

作者：

Cagnin, Stefano ^{[1
,2
]}

Caraballo, Marcelo ^{[3
]}

Guiducci, Carlotta ^{[4
,5
]}

Martini, Paolo ^{[1
,2
]}

Ross, Marty ^{[3
]}

SantaAna, Mark ^{[3
]}

Danley, David ^{[3
]}

West, Todd ^{[3
]}

Lanfranchi, Gerolamo ^{[1
,2
]}

机构：

[1] Univ Padua, CRIBI Biotechnol Ctr, I-35121 Padua, Italy

[2] Univ Padua, Dept Biol, I-35121 Padua, Italy

[3] CombiMatrix Corp, Mukilteo, WA 98275 USA

[4] Univ Bologna, DEIS, I-40136 Bologna, Italy

[5] Ecole Polytech Fed Lausanne, Inst Bioengn, IBI, CH-1015 Lausanne, Switzerland

来源：

SENSORS | 2009年 / 9卷 / 04期

关键词：

DNA chip; Electrochemical DNA detection; Biosensors; Microarray; SURFACE-PLASMON RESONANCE; GENETICALLY-MODIFIED ORGANISMS; DIFFERENTIAL GENE-EXPRESSION; QUARTZ-CRYSTAL-MICROBALANCE; POLYMERASE-CHAIN-REACTION; LABEL-FREE DETECTION; OLIGONUCLEOTIDE ARRAYS; ELECTRONIC DETECTION; SIGNAL AMPLIFICATION; SENSITIVE DETECTION;

D O I：

10.3390/s90403122

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA microarrays are an important tool with a variety of applications in gene expression studies, genotyping, pharmacogenomics, pathogen classification, drug discovery, sequencing and molecular diagnostics. They are having a strong impact in medical diagnostics for cancer, toxicology and infectious disease applications. A series of papers have been published describing DNA biochips as alternative to conventional microarray platforms to facilitate and ameliorate the signal readout. In this review, we will consider the different methods proposed for biochip construction, focusing on electrochemical detection of DNA. We also introduce a novel single-stranded DNA platform performing high-throughput SNP detection and gene expression profiling.

引用

页码：3122 / 3148

页数：27

共 162 条

[1] DNA single-base mismatch study with an electrochemical enzymatic genosensor [J].